Outside-inside distribution and translocation of lysophosphatidylcholine in phosphatidylcholine vesicles as determined by 13C-NMR using (N-13CH3)-enriched lipids
Outside-inside distribution and translocation of lysophosphatidylcholine in phosphatidylcholine vesicles as determined by 13C-NMR using (N-13CH3)-enriched lipids
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使用富含 (N-13CH3) 的脂质通过 13C-NMR 测定磷脂酰胆碱囊泡中溶血磷脂酰胆碱的外向内分布和易位
DOI：
10.1016/0005-2736(77)90263-2
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发表时间：
1977
期刊：
影响因子：
--
通讯作者：
L. M. Deenen
中科院分区：
文献类型：
--
作者：
B. D. Kruyff;A. Besselaar;L. M. Deenen
1.1.使用 13 C NMR 和 Dy3+ 作为不可渗透的位移试剂，用 (N-13CH3) 标记的脂质测量混合超声处理囊泡中棕榈酰溶血磷脂酰胆碱和二油酰磷脂酰胆碱的外向内分布。2.2．棕榈酰溶血磷脂酰胆碱优先位于囊泡膜的外层。囊泡中胆固醇的掺入减少了溶血磷脂酰胆碱不对称的程度。3.3。添加到二油酰磷脂酰胆碱囊泡中的棕榈酰溶血磷脂酰胆碱掺入囊泡的外单层中。即使 40 小时后，在内单层中也能检测到不到 2% 的溶血磷脂酰胆碱。由于在共声化的囊泡中，17% 的溶血磷脂酰胆碱存在于内部单层中，因此可以得出结论，溶血磷脂酰胆碱穿过这些囊泡的脂质双层的跨膜运动是一个极其缓慢的过程。
1.1. The outside-inside distribution of palmitoyl lysophosphatidylcholine and dioleoyl phosphatidylcholine in mixed sonicated vesicles is measured with (N-13CH3)-labelled lipids using13C NMR and Dy3+as an impermeable shift reagent.2.2. Palmitoyl lysophosphatidylcholine is preferentially localised in the outside layer of the vesicle membrane. Incorporation of cholesterol in the vesicle diminishes the extent of lysophosphatidylcholine asymmetry.3.3. Palmitoyl lysophosphatidylcholine added to dioleoyl phosphatidylcholine vesicles is incorporated in the outer monolayer of the vesicle. Even after 40 h less than 2% of the lysophosphatidylcholine could be detected in the inner monolayer. Since in the cosonicated vesicles 17% of the lysophosphatidylcholine is present in the inner monolayer it can be concluded that the transmembrane movement of lysophosphatidylcholine across the lipid bilayer of these vesicles is an extremely slow process.