Demonstration of lateral transmission of scrapie between sheep kept under natural conditions using lymphoid tissue biopsy
Demonstration of lateral transmission of scrapie between sheep kept under natural conditions using lymphoid tissue biopsy
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DOI：
10.1016/j.rvsc.2003.11.007
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发表时间：
2004-06-01
影响因子：
2.4
通讯作者：
Tongue, S
中科院分区：
文献类型：
--
作者：
Ryder, S;Dexter, G;Tongue, S
将无痒病的成年羊引入专门维持以最大限度地提高痒病感染的羊群。该羊群中高易感VRQ/VRQ基因型的本地出生羊表现出高效率的传播，100%的感染率证明了这一点，死亡年龄不到2岁。引入羊的感染是通过扁桃体和烟膜淋巴组织活检确定的。对这些羊的后代进行监测，并使用常规诊断方法通过脑部检查确认临床疾病。新西兰原产的成年羊被感染，表明发生了横向传播。引进母羊所生的羔羊被感染并死亡的年龄与本地母羊所生的羔羊相同，这与痒病向羔羊的横向传播一致。虽然在本研究中不能完全排除母系传播，但数据一致表明，横向传播是最重要的传播方式，导致在该羊群中观察到的瘙痒病高发病率。英国皇家版权所有(C) 2004, Elsevier Ltd.出版版权所有。
Scrapic free adult sheep were introduced to a sheep flock specifically maintained to maximise scrapie infection. Native born sheep of the highly susceptible VRQ/VRQ genotype in this flock show highly efficient transmission, evidenced by 100% infection, with an age at death of less than 2 years. Infection in introduced sheep was identified by biopsy of tonsilar and nictitating membrane lymphoid tissue. Progeny of these sheep were monitored and clinical disease confirmed by examination of the brain using routine diagnostic methods. Naive sheep of New Zealand origin introduced to the flock in adulthood became infected, demonstrating that lateral transmission had occurred. Lambs born to introduced ewes became infected and died at the same age as lambs born to native ewes, consistent with lateral transmission of scrapie to lambs. Although maternal transmission cannot be totally excluded for the lambs in this study, the data are consistent with lateral transmission being the most important means of spread leading to the high incidence of scrapie observed in this flock. Crown Copyright (C) 2004 Published by Elsevier Ltd. All rights reserved.