Large-scale identification of expressed sequence tags involved in rice and rice blast fungus interaction
Large-scale identification of expressed sequence tags involved in rice and rice blast fungus interaction
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DOI：
10.1104/pp.104.055624
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发表时间：
2005-05-01
期刊：
影响因子：
7.4
通讯作者：
Wang, GL
中科院分区：
文献类型：
--
作者：
Jantasuriyarat, C;Gowda, M;Wang, GL
为了更好地了解水稻对稻瘟病菌(Magnaporthe Grisea)防御反应的分子基础，利用大规模表达序列标签(EST)测序方法对水稻(Oryza Sativa)早期侵染相关基因进行了筛选。从接种后6h和2 4h的抗病、部分抗病和感病6种条件下采集的病叶组织构建了6个cDNA文库。使用未接种的叶片和来自病斑模拟突变体Spl11的叶片构建了另外两个文库。从8个文库中共获得68920条EST。聚类和组装分析从10,934个重叠群和2,636个单体中获得了13,570个独特序列。基因功能分类表明，42%的EST被预测具有可能的基因功能。病原菌挑战文库与未接种对照文库的比较表明，在防御和信号转导机制以及细胞周期控制、细胞分裂和染色体分裂等功能类别中的基因百分比增加。此外，等级聚类分析根据八个文库的疾病反应对其进行了分组。与KOME全长文库相比，我们的文库共鉴定出7748个新的和独特的EST。有趣的是，我们发现水稻EST与高粱EST的亲缘关系比与大麦、小麦和玉米EST的亲缘关系更近。本研究收集的大量水稻EST序列为进一步研究水稻的防御反应奠定了坚实的基础，为水稻功能基因组学研究提供了有用的公共基因组资源。
To better understand the molecular basis of the defense response against the rice blast fungus ( Magnaporthe grisea), a large- scale expressed sequence tag ( EST) sequencing approach was used to identify genes involved in the early infection stages in rice ( Oryza sativa). Six cDNA libraries were constructed using infected leaf tissues harvested from 6 conditions: resistant, partially resistant, and susceptible reactions at both 6 and 24 h after inoculation. Two additional libraries were constructed using uninoculated leaves and leaves from the lesion mimic mutant spl11. A total of 68,920 ESTs were generated from 8 libraries. Clustering and assembly analyses resulted in 13,570 unique sequences from 10,934 contigs and 2,636 singletons. Gene function classification showed that 42% of the ESTs were predicted to have putative gene function. Comparison of the pathogen- challenged libraries with the uninoculated control library revealed an increase in the percentage of genes in the functional categories of defense and signal transduction mechanisms and cell cycle control, cell division, and chromosome partitioning. In addition, hierarchical clustering analysis grouped the eight libraries based on their disease reactions. A total of 7,748 new and unique ESTs were identified from our collection compared with the KOME full- length cDNA collection. Interestingly, we found that rice ESTs are more closely related to sorghum ( Sorghum bicolor) ESTs than to barley ( Hordeum vulgare), wheat ( Triticum aestivum), and maize ( Zea mays) ESTs. The large cataloged collection of rice ESTs in this study provides a solid foundation for further characterization of the rice defense response and is a useful public genomic resource for rice functional genomics studies.