Strictness of the Centrifugal Location of Bundle Sheath Chloroplasts in Different NADP-ME Type C4 Grasses
Strictness of the Centrifugal Location of Bundle Sheath Chloroplasts in Different NADP-ME Type C4 Grasses
复制标题
DOI：
10.1626/pps.6.274
复制
发表时间：
2003-01
影响因子：
2.5
通讯作者：
Yojiro Taniguchi;M. Taniguchi;M. Kawasaki;H. Miyake
中科院分区：
文献类型：
--
作者：
Yojiro Taniguchi;M. Taniguchi;M. Kawasaki;H. Miyake
C4植物具有许多吸引作物的性状，但其结构和功能关系复杂。C4植物的束鞘细胞（BSC）叶绿体位置（离心式或向心式）不同。采用光镜和电镜研究了不同光照强度对4种NADP苹果酸酶（NADP- me）型禾草（玉米、紫锥草、双色高粱和羊耳草）BSC叶绿体离心位置的影响。此外，我们还考察了叶肉细胞的颗粒发育程度，探讨了叶肉细胞叶绿体位置与叶肉细胞对还原性的依赖之间的关系。研究了强光（HL） （600 μ mol m-2s-1）、弱光（LL） （2.5 μ mol m-2s-1）和黑暗条件下生长的BSC叶绿体的位置，并计算了每个颗粒中类囊体的数量。尽管玉米BSC叶绿体在所有光照条件下都保持离心位置，但在强光和黑暗条件下，其他物种的离心位置受到干扰。在高温胁迫下生长的植物，BSC叶绿体的颗粒发育受到抑制，但双色花和青花花受抑制的程度较其他两种植物更为明显。这些结果表明，NADP-ME型C4禾草种间BSC叶绿体离心定位的严格性存在差异，且这种严格性与BSC叶绿体的颗粒发育程度无关。
Abstract C4 plants have many attractive traits for crops, but their structural and functional relationships are complicated. C4 plants are different in bundle sheath cell (BSC) chloroplast location (centrifugal or centripetal) among species. The effects of light intensity on the centrifugal location of BSC chloroplasts were investigated in four grass species of NADP malic enzyme (NADP-ME) type (Zea mays, Echinochloa utilis, Sorghum bicolorand Eriachne aristidea)by light and electron microscopy. Furthermore, the degree of granal development was examined to investigate the relation between BSC chloroplast location and dependence of BSC chloroplasts on the reducing power of mesophyll cells. We investigated BSC chloroplast location grown under high intensity light (HL) (600 μ mol m-2s-1), low intensity light (LL) (2.5 μ mol m-2s-1) and dark conditions and counted the number of granal thylakoids per granum. Although BSC chloroplasts of maize maintained the centrifugal position under all light conditions, the centrifugal location of other species was disturbed under LL and in the dark. Granal development in BSC chloroplasts in the plants grown under HL was suppressed, although the suppression in Z maysand S. bicolorwas more prominent than in other two species. These findings indicate that there is a difference in the strictness of centrifugal location of BSC chloroplasts among NADP-ME type C4 grass species and the strictness had no relation to the degree of granal development in BSC chloroplasts.