Metabolite profiles of icariin in rat plasma by ultra-fast liquid chromatography coupled to triple-quadrupole/time-of-flight mass spectrometry
Metabolite profiles of icariin in rat plasma by ultra-fast liquid chromatography coupled to triple-quadrupole/time-of-flight mass spectrometry
复制标题
通过超快液相色谱联用三重四极杆/飞行时间质谱法研究大鼠血浆中淫羊藿苷的代谢谱
DOI：
10.1016/j.jpba.2012.03.053
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发表时间：
2012-07-01
影响因子：
3.4
通讯作者：
Jia, Xiao-Bin
中科院分区：
文献类型：
--
作者：
Qian, Qian;Li, Song-Lin;Jia, Xiao-Bin
本研究定性研究了淫羊藿苷在大鼠血浆中的代谢谱，并提出了淫羊藿苷可能的代谢途径。口服淫羊藿苷后，采集大鼠血浆样品，进行蛋白沉淀预处理。将预处理后的样品注入以0.1%甲酸水和0.1%甲酸乙腈为流动相的Venusil ASB-C18柱中，采用超快速液相色谱-飞行时间质谱（UFLC-TOF/MS）进行检测。代谢产物共19种，分别为：icariside I （M1）、icaritin （M2）、去甲基icaritin及其异构体（M3-M6）、icaritin-3- o - glua （M7）、icaritin-7- o - glua （M8）、icaritin II及其异构体（M9和M10）、icaritin-3,7-二- o - glua （M11）、1,3-异戊二醇icaritin及其异构体（M12、M13和M18）、1,3-异戊二醇icariide II （M14）、烯丙醇icaritin及其异构体（M15和M16）。检测并初步鉴定了1,3-异戊二烯icariside II （M17）和icaratin -3- o - rhaa -7- o - glua (M19)，其中M7、M8和M12-M18等9个为首次报道。血浆代谢谱显示，异黄酮的葡萄糖醛酸缀合物和类黄酮苷元是淫羊藿苷的主要循环形式。(C) 2012 Elsevier B.V.版权所有
In this work, the metabolite profiles of icariin in rat plasma were qualitatively investigated, and the possible metabolic pathways of icariin were subsequently proposed. After oral administration of icariin, rat plasma samples were collected and pretreated by protein precipitation. Then these pretreated samples were injected into a Venusil ASB-C18 column with mobile phase consisted of 0.1% formic acid water and 0.1% formic acid acetonitrile and detected by Ultra fast liquid chromatography coupled to time-of-flight mass spectrometry (UFLC-TOF/MS). A total of 19 metabolites, namely, icariside I (M1), icaritin (M2), desmethylicaritin and its isomer (M3-M6), icaritin-3-O-gluA (M7), icaritin-7-O-gluA (M8), icariside II and its isomer (M9 and M10), icaritin-3,7-di-O-gluA (M11), 1,3-isoprene alcohol icaritin and its isomer (M12, M13 and M18), 1,3-isoprene alcohol icariside II (M14), allylic alcohol icaritin and its isomer (M15 and M16), 1,3-isoprene icariside II (M17) and icaritin-3-O-rha-7-O-gluA (M19) were detected and tentatively identified, and 9 of them, including M7 and M8 and M12-M18 were reported for the first time. The metabolites profiles in plasma revealed that glucuronide conjugates of isoflavonoids and flavonoid aglycones were the major circulating forms of icariin. (C) 2012 Elsevier B.V. All rights reserved.