Limitation of infarct size in the rabbit by ischaemic preconditioning is reversible with glibenclamide.
Limitation of infarct size in the rabbit by ischaemic preconditioning is reversible with glibenclamide.
复制标题
格列本脲可逆转缺血预处理对家兔梗塞面积的限制。
DOI：
10.1093/cvr/27.4.617
复制
发表时间：
1993
影响因子：
10.8
通讯作者：
Shebuski Rj
中科院分区：
文献类型：
--
作者：
C. Toombs;Teresa L. Moore;Shebuski Rj
目的
目的是确定缺血预处理是否通过ATP敏感性钾（KATP）通道在缺血期间的作用发生，以及这些通道的药理学拮抗作用是否可以逆转预处理的保护作用。
方法
31只新西兰白色兔行冠状动脉阻断和再灌注。对照组动物仅进行缺血（30分钟）和再灌注（120分钟）。将研究动物分为三个实验组：（1）在30 min缺血前接受静脉注射格列本脲（0.3 mg.kg-1）的动物;（2）在30 min缺血前接受5 min预处理缺血和10 min再灌注的动物;或（3）在30 min缺血前接受格列本脲预处理的动物。用印度墨水和四氮唑染色观察所产生的梗死和危险心肌，并使用计算机辅助面积测量法进行定量。
结果
与非预处理对照组[39.8（2.1）%]相比，预处理的家兔显示梗死面积减少63%[风险区域的14.8（SEM 2.2）%]。当在存在格列本脲的情况下对家兔进行预处理时，使用格列本脲剂量（其本身不改变坏死[37.7（5.4）%]）逆转了保护作用[31.3（5.1）%]。
结论
与格列本脲联合给药时，通过缺血预处理使家兔心肌体积缩小是可逆的。
OBJECTIVE
The aim was to determine whether ischaemic preconditioning occurs through the actions of ATP sensitive potassium (KATP) channels during ischaemia and whether pharmacological antagonism of these channels can reverse the protective effect of preconditioning.
METHODS
31 New Zealand White rabbits were instrumented for coronary occlusion and reperfusion. Control animals were subjected to ischaemia (30 min) and reperfusion (120 min) only. Study animals were divided into three experimental groups: (1) those receiving intravenous glibenclamide (0.3 mg.kg-1) prior to the 30 min ischaemia; (2) those receiving 5 min preconditioning ischaemia and 10 min reperfusion prior to the 30 min ischaemia; or (3) those receiving preconditioning in the presence of glibenclamide prior to the 30 min ischaemia. The resulting infarct and myocardium at risk were visualised with Indian ink and tetrazolium staining and quantified using computer assisted planimetry.
RESULTS
Rabbits which were preconditioned showed a 63% reduction in infarct size [14.8(SEM 2.2)% of risk region] in comparison to non-preconditioned controls [39.8(2.1)%]. When rabbits were preconditioned in the presence of glibenclamide the protection was reversed [31.3(5.1)%] using a dose of glibenclamide which by itself did not alter necrosis [37.7(5.4)%].
CONCLUSIONS
Infarct size reduction in the rabbit via ischaemic preconditioning is reversible with the coadministration of glibenclamide.