The motility of Chara corallina myosin was inhibited reversibly by 2,3-butanedione monoxime (BDM)
The motility of Chara corallina myosin was inhibited reversibly by 2,3-butanedione monoxime (BDM)
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DOI：
10.1093/pcp/pch154
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发表时间：
2004-09-01
影响因子：
4.9
通讯作者：
Yamamoto, K
中科院分区：
文献类型：
--
作者：
Funaki, K;Nagata, A;Yamamoto, K
我们研究了2，3-丁二酮单肟（BDM）对轮藻细胞质流动和从同一种植物制备的肌球蛋白的运动性的影响，以检查该试剂是否真的影响植物XI类肌球蛋白。发现BDM在非常相似的浓度范围（10-100 mM）下抑制细胞质流动和肌球蛋白的运动性。BDM直接引入到无液泡膜的细胞也抑制细胞质流。这些结果表明，BDM对细胞质流动的影响是通过肌球蛋白而不是通过离子通道来实现的，至少在轮藻中是这样的，尽管需要很高的浓度。
We studied the effects of 2,3-butanedione monoxime (BDM) on the cytoplasmic streaming of Chara corallina and on the motility of myosin prepared from the same plant to examine whether this reagent really affects the plant class XI myosin. It was found that BDM inhibited both cytoplasmic streaming and the motility of myosin at a very similar concentration range (10-100 mM). BDM introduced directly into tonoplast-free cells also inhibited cytoplasmic streaming. These results suggested that effect of BDM on cytoplasmic streaming was exerted through myosin and not through ion channels at least in Chara corallina, though a very high concentration of BDM was required.