MRC2020: improvements to Ximdisp and the MRC image-processing programs.
MRC2020: improvements to Ximdisp and the MRC image-processing programs.
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作者：
描述了MRC图像处理软件包的更新。它包括对Ximdisp显示程序的改进，用于螺旋组件图像或倾斜对单粒子低温电磁图像。单粒子电子冷冻显微镜(CryoEM)在过去十年中的巨大成功涉及到强大的新计算机程序和程序包的开发，这些程序和程序包指导用户遵循推荐的处理工作流程，其中开发人员所做的智慧和选择帮助每个人，特别是新用户，获得出色的结果。执行新颖的、非标准的或不寻常的图像处理步骤组合的能力有时会因标准程序的便利性而受到影响。一些较老的程序编写时具有很大的灵活性，现在仍然非常有价值。其中，用于二维晶体和螺旋处理确定结构的原始MRC图像处理程序以及通用实用程序如Ximdisp、LABEL、IMEDIT和TWIFILE仍然可用。这项工作描述了可从CCP-EM免费获得的MRC软件包(MRC2020)的更新版本。它包括新的功能和改进，如对MRC格式的扩展，保留了该包的多功能性，使其特别适用于在CryoEM中测试新的计算程序。
An update to the MRC image-processing software package is described. It includes improvements to the Ximdisp display program for use on images of helical assemblies or tilt-pair single-particle cryoEM images. The great success of single-particle electron cryo-microscopy (cryoEM) during the last decade has involved the development of powerful new computer programs and packages that guide the user along a recommended processing workflow, in which the wisdom and choices made by the developers help everyone, especially new users, to obtain excellent results. The ability to carry out novel, non-standard or unusual combinations of image-processing steps is sometimes compromised by the convenience of a standard procedure. Some of the older programs were written with great flexibility and are still very valuable. Among these, the original MRC image-processing programs for structure determination by 2D crystal and helical processing alongside general-purpose utility programs such as Ximdisp, label, imedit and twofile are still available. This work describes an updated version of the MRC software package (MRC2020) that is freely available from CCP-EM. It includes new features and improvements such as extensions to the MRC format that retain the versatility of the package and make it particularly useful for testing novel computational procedures in cryoEM.
影响因子：
5.7
作者：
Zhang, Qinfen;Dai, Xinghong;Cong, Yao;Zhang, Junjie;Chen, Dong-Hua;Dougherty, Matthew T.;Wang, Jiangyong;Ludtke, Steven J.;Schmid, Michael F.;Chiu, Wah
通讯作者：
Chiu, Wah