A kinetic study of glucagon-like peptide-1 and glucagon-like peptide-2 truncation by dipeptidyl peptidase IV, in vitro
A kinetic study of glucagon-like peptide-1 and glucagon-like peptide-2 truncation by dipeptidyl peptidase IV, in vitro
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DOI：
10.1016/s0006-2952(02)01415-6
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发表时间：
2002-12-15
影响因子：
5.8
通讯作者：
De Meester, I
中科院分区：
文献类型：
--
作者：
Lambeir, AM;Proost, P;De Meester, I
胰高血糖素样肽-1（GLP-1）和胰高血糖素样肽-2（GLP-2）的体内失活与外肽酶二肽基肽酶TV（DPP IV）对其N-末端二肽的蛋白水解去除有关。先前的研究表明，GLP-1的体内代谢比GLP-2快得多。本文研究了DPP IV对GLP-2和GLP-1的体外截短作用。与GLP-1相比，GLP-2观察到的较慢的转化速率是由于催化速率常数降低约10倍。将DPP IV对胰高血糖素样肽的选择性与在相同条件下使用相同酶源获得的其他天然底物的数据进行比较。(C)2002年爱思唯尔科技有限公司All rights reserved.
In vivo inactivation of glucagon-like peptide-1 (GLP-1) and glucagon-like peptide-2 (GLP-2) was found to be associated with the proteolytic removal of their N-terminal dipeptide by the ectopeptidase dipeptidyl peptidase TV (DPP IV). Previous studies suggested that the in vivo metabolism of GLP-1 is much faster than that of GLP-2. In this paper, we investigated the in vitro truncation of GLP-2 and GLP-1 by DPP IV. The slower conversion rate observed for GLP-2 compared to GLP-1 was due to an approximately 10-fold reduction in catalytic rate constant. The selectivity of DPP IV for the glucagon-like peptides was compared with data obtained for other natural substrates using the same enzyme source in identical conditions. (C) 2002 Elsevier Science Inc. All rights reserved.