Referenced luminescent sensing and imaging with digital color cameras: A comparative study
Referenced luminescent sensing and imaging with digital color cameras: A comparative study
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DOI：
10.1016/j.snb.2012.11.041
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发表时间：
2013-02-01
影响因子：
8.4
通讯作者：
Schaeferling, Michael
中科院分区：
文献类型：
--
作者：
Meier, Robert J.;Fischer, Lorenz H.;Schaeferling, Michael
我们对光学化学传感器的不同成像技术进行了比较研究，目的是评估红绿蓝（RGB）彩色相机在定量分析中的效用。采用一种用于感应气压的发光薄膜（通过氧猝灭）作为模型系统，并采用四种荧光成像方法进行校准，包括强度成像、参考强度成像、寿命成像和基于RGB的常规数字彩色相机成像。结果在标准偏差、横向信号均匀性和分辨率方面进行了比较。在相同的实验条件下，将成像方法应用于传感器薄膜，以确保结果的可比性。(c) 2012 Elsevier B.V.版权所有
We have performed a comparative study on different imaging techniques for optical chemical sensors with the aim to assess the utility of red-green-blue (RGB) color cameras for quantitative analysis. A luminescent film for sensing barometric pressure (via quenching by oxygen) was used as a model system and calibrated by four fluorescence imaging methods including intensity imaging, referenced intensity imaging, lifetime imaging, and RGB based imaging using a customary digital color camera. The results are compared with respect to standard deviations, lateral signal homogeneity, and resolution. The imaging methods were applied to the sensor film under identical experimental conditions in order to ensure comparable results. (c) 2012 Elsevier B.V. All rights reserved.