Cloning, phylogenetic analysis and postnatal expression of oligopeptide transporter PepT1 in gastrointestinal tract of kid goats receiving supplemental feed or pasture
Cloning, phylogenetic analysis and postnatal expression of oligopeptide transporter PepT1 in gastrointestinal tract of kid goats receiving supplemental feed or pasture
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补充饲料或牧草小山羊胃肠道中寡肽转运蛋白 PepT1 的克隆、系统发育分析和产后表达
DOI：
10.1139/cjas-2019-0155
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发表时间：
2020
影响因子：
1
通讯作者：
Karen A.Beauchemin
中科院分区：
文献类型：
--
作者：
Tao Ran;Hengzhi Li;Yong Liu;Chuanshe Zhou;Zhixiong He;Tan Zhiliang;W.Z.Yang;Karen A.Beauchemin
本研究旨在克隆H+依赖性寡肽转运蛋白PepT 1的cDNA，并研究不同生理发育（哺乳期、断奶期和断奶后）和饲养制度（补饲与放牧）对小山羊肽转运能力的影响。克隆了一个2395 bp的pept 1基因cDNA序列（GenBank：MH 308024），系统发育分析表明，该基因与绵羊和牛的PepT 1基因具有高度的同源性和结构相似性。pept 1在小山羊出生后即刻和发育过程中在整个胃肠道中表达。pept 1在哺乳期除空肠中段外的各肠段的相对丰度均呈下降趋势，而在断奶后的大部分小肠段中的表达随日龄的增加而增加，此后保持稳定。空肠中段是主要的表达部位，也可能是主要的肽吸收部位。补饲增强了pept 1的表达，因为与放牧相比，补饲增加了蛋白质的摄入。在大多数节段中未观察到饲养制度×年龄的交互作用;在哺乳期表达与年龄相关，在断奶和断奶后表达与饮食相关，表明饲养制度和年龄对pept 1表达有独立影响。提示PepT 1在新生山羊蛋白质营养中起重要作用，其表达受饲养制度的影响。
This study aimed to clone the cDNA of PepT1, an H+-dependent oligopeptide transporter, from kid goats and examine effects of physiological development (suckling, weaning, and post-weaning) of the animal and feeding system (supplemental feeding vs. grazing) on peptide transport capability. A 2395 bp cDNA sequence ofpept1(GenBank: MH308024) was cloned and phylogenetic analysis revealed a high homology and structure similarity with PepT1 of sheep and cattle. Thepept1was expressed throughout the gastrointestinal tract of kid goats immediately after birth and during development. Relative abundance ofpept1decreased in all segments except the middle-jejunum during suckling, whereas its expression in most segments of small intestine increased with age after weaning and remained stable thereafter. Middle-jejunum was the predominant expression site and probably the main peptide absorption site. Supplemental feeding enhancedpept1expression because it increased protein intake compared with grazing. No feeding system × age interaction was observed in most segments; the expression was age related during suckling and diet related during weaning and post-weaning, indicating that feeding system and age had independent effects onpept1expression. These results indicate that PepT1 plays an important role for protein nutrition in neonatal goats, and its expression can be affected by feeding system.