Expression Profiles of Exosomal MicroRNAs from HEV- and HCV-Infected Blood Donors and Patients: A Pilot Study
Expression Profiles of Exosomal MicroRNAs from HEV- and HCV-Infected Blood Donors and Patients: A Pilot Study
复制标题
DOI：
10.3390/v12080833
复制
发表时间：
2020-08-01
期刊：
影响因子：
4.7
通讯作者：
Petrik, Juraj
中科院分区：
文献类型：
--
作者：
McGowan, Karen;Simpson, Kenneth J.;Petrik, Juraj
外泌体似乎在丙型肝炎病毒（HCV）和戊型肝炎病毒（HEV）感染中发挥重要作用，通过保护其货物免受宿主免疫反应的影响，microrna是关键的外泌体成分。对于它们是否参与混合HCV/HEV感染或在感染的早期阶段，例如在无症状献血者中，所知甚少。为了获得初步数据，我们比较了4名HCV rna阳性、HEV rna阳性和阴性献血者和4名患者（其中1名罕见的HCV/HEV合并感染患者）的外泌体microRNA表达谱。采用沉淀和密度梯度离心相结合的方法从血清中纯化外泌体，并使用Taqman阵列卡分析外泌体microRNA。在33个解除调控的mirna中，miR-885-5p和miR-365在HCV BD中上调，miR-627-5p在HCV BD中下调，miR-221在HCV患者和BD中下调。在HEV感染中，miR-526b出现特异性下调。六种mirna （miR-628-3p, miR-194, miR-151-3p, miR-512-3p， miR-335和miR-590）表明可能参与这两种感染。关于HEV/HCV共感染患者抗病毒治疗前后外泌体microRNA谱的首次初步数据显示，77个microRNA上调，43个microRNA下调，这些microRNA在这些病毒感染中的潜在作用有待进一步研究。
Exosomes seem to play an important role in hepatits C virus (HCV) and hepatitis E virus (HEV) infection by shielding their cargo from the host immune responses, with microRNAs being key exosomal components. Little is known about their involvement in a mixed HCV/HEV infection or at the early stages of infection, such as in asymptomatic blood donors (BDs). To obtain preliminary data, we have compared the exosomal microRNA expression profiles in four each of HCV RNA-positive, HEV RNA-positive and negative blood donors and four patients, one of whom was a rare patient with HCV/HEV co-infection. Exosomes were purified from sera by a combination of a precipitation and density gradient centrifugation and exosomal microRNA was analysed using Taqman array cards. Out of 33 deregulated miRNAs, miR-885-5p and miR-365 were upregulated in HCV BDs, miR-627-5p was downregulated in HCV BD and miR-221 was downregulated in HCV patients and BDs. In HEV infection, miR-526b appeared specifically downregulated. Six miRNAs (miR-628-3p, miR-194, miR-151-3p, miR-512-3p, miR-335 and miR-590) indicated a potential involvement in both infections. First time preliminary data on pre- and post-antiviral treatment exosomal microRNA profiles of the HEV/HCV co-infected patient revealed a pool of 77 upregulated and 43 downregulated miRNAs to be further investigated for their potential roles in these viral infections.