INFRARED MULTIPHOTON DISSOCIATION OF LARGE MULTIPLY-CHARGED IONS FOR BIOMOLECULE SEQUENCING
INFRARED MULTIPHOTON DISSOCIATION OF LARGE MULTIPLY-CHARGED IONS FOR BIOMOLECULE SEQUENCING
复制标题
DOI：
10.1021/ac00090a004
复制
发表时间：
1994-09-15
影响因子：
7.4
通讯作者：
MCLAFFERTY, FW
中科院分区：
文献类型：
--
作者：
LITTLE, DP;SPEIR, JP;MCLAFFERTY, FW
用电喷雾电离的高分辨率串联傅里叶变换质谱对大生物分子的结构进行表征和验证，关键取决于用于将产生的多电荷离子片段化的技术。红外多光子解离（IRMPD）的电离蛋白质大的碳酸酐酶（29 kDa）产生的片段信息类似，但有价值的补充，其他解离技术。IRMPD在轴上产生产物离子，在进一步的阶段提供有效的解离;泛素（8.6 kDa）的MS（3）产生11个新的序列离子。蛋白质离子解离的最佳照射时间变化超过6倍，寡核苷酸的时间远低于此，这可能是由于具有P-O伸缩频率的光子共振。IRMPD提供比碰撞活化解离大得多的选择性，并且似乎产生少得多的质量歧视和解离更稳定的离子。一种从激光束中去除形成时的产物离子的技术应该将目前的效率提高30- 80%。
Characterization and verification of the structures of large biomolecules with high-resolution tandem Fourier transform mass spectrometry with electrospray ionization is critically dependent on the technique used to fragment the multiply charged ions produced. infrared multiphoton dissociation (IRMPD) of ionized proteins as large as carbonic anhydrase (29 kDa) yields fragment information similar to, but with valuable additions to, that of other dissociation techniques. IRMPD yields product ions on-axis, providing efficient dissociation in further stages; MS(3) of ubiquitin (8.6 kDa) yields 11 new sequence ions. Optimum irradiation times for protein ion dissociation vary by more than a factor of 6, with times for oligonucleotides far lower, possibly due to photon resonance with a P-O stretching frequency. IRMPD provides far greater selectivity than collisionally activated dissociation and also appears to yield much less mass discrimination and to dissociate much more stable ions. A technique to remove product ions on formation from the laser beam should improve the present efficiencies of 30-80%.