DETECTION OF PICOMOLE LEVELS OF HYDROPEROXIDES USING A FLUORESCENT DICHLOROFLUORESCEIN ASSAY
DETECTION OF PICOMOLE LEVELS OF HYDROPEROXIDES USING A FLUORESCENT DICHLOROFLUORESCEIN ASSAY
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DOI：
10.1016/0003-2697(83)90270-1
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发表时间：
1983-01-01
影响因子：
2.9
通讯作者：
AMES, BN
中科院分区：
文献类型：
--
作者：
CATHCART, R;SCHWIERS, E;AMES, BN
本文描述了一种高灵敏度的荧光法，用于皮摩尔水平上胆固醇、脂质和其它氢过氧化物的定量测定。该方法是基于在温和条件下，过氧化氢和高铁血红素氧化二氯荧光素生成荧光二氯荧光素。在添加的氢过氧化物和产生的二氯荧光素之间观察到1：1的化学计量。由于内过氧化物（例如，PGH2 [前列腺素H2]）在测定中不发生反应，不会干扰脂质氢过氧化物的测定。
A highly sensitive fluorometric method for the quantitation of cholesterol, lipid and other hydroperoxides at the picomole level is described. The method is based on the oxidation of dichlorofluoroscin to the fluorescent dichlorofluoroscein by hydroperoxide and hematin under mild conditions. A 1:1 stoichiometry is observed between the hydroperoxide added and the dichlorofluoroscein produced. Since endoperoxides (e.g., PGH2 [prostaglandin H2]) do not react in the assay, they do not interfere in the determination of lipid hydroperoxides.