Comparison of preimplantation developmental competence after mouse oocyte growth and development in vitro and in vivo
Comparison of preimplantation developmental competence after mouse oocyte growth and development in vitro and in vivo
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DOI：
10.1016/s0093-691x(97)00413-5
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发表时间：
1998-01-15
期刊：
影响因子：
2.8
通讯作者：
O'Brien, MJ
中科院分区：
文献类型：
--
作者：
Eppig, JJ;O'Brien, MJ
卵母细胞培养系统对于卵母细胞发育基本机制的实验分析是必不可少的，并且最终将在农业、临床和野生动物保护中得到广泛应用。本文综述了利用腔前卵泡卵母细胞颗粒细胞复合物体外培养和发育小鼠卵母细胞的进展。从12日龄小鼠的腹前（次）卵泡中分离卵母细胞-颗粒细胞复合物，体外培养10 d，然后成熟并体外受精。将这些卵母细胞的发育能力与体内培养的卵母细胞和22日龄小鼠体外成熟受精的卵母细胞进行比较。体外培养的卵母细胞没有达到与体内培养的卵母细胞相同的大小。然而，当卵母细胞在含有胎牛血清的培养基中生长时，其着床前发育能力与体内生长的卵母细胞相当。令人惊讶的是，当卵母细胞在体外培养时，每只动物产生的囊胚比在体内培养时多。卵母细胞大小与着床前发育能力或每个囊胚细胞数均无相关性。在无血清培养基中生长的卵母细胞与在有血清培养基中生长的卵母细胞的发育能力不同。最后，描述和讨论了唯一一只体外卵母细胞发育完全后出生的成年动物的健康状况。(C) 1998年Elsevier Science Inc。
Culture systems for oocytes are essential for the experimental analysis of the basic mechanisms of oocyte development and, moreover, they will eventually find wide application in agriculture, the clinic, and wildlife preservation. Here, progress in mouse oocyte growth and development in vitro using oocyte-granulosa cell complexes from preantral follicles is reviewed. Oocyte-granulosa cell complexes were isolated from preantral (secondary) follicles of 12 day old mice, grown in vitro for 10 days, then matured and fertilized in vitro. The developmental competence of these oocytes was compared with oocytes grown in vivo and isolated from 22 day old mice, then matured and fertilized in vitro. in vitro-grown oocytes did not achieve the same size as their in vivo-grown counterparts. However, when oocytes were grown in medium containing fetal bovine serum, their preimplantation developmental competence was equivalent to that of in vivo-grown oocytes. Surprisingly, more blastocysts per animal were produced when oocytes were grown in vitro than in vivo. There was no correlation between oocyte size and either preimplantation developmental competence or number of cells per blastocyst. Oocytes grown in serum-free medium did not achieve the same developmental competence as oocytes grown in medium supplemented with serum. Lastly, the health status as an adult of the only animal born after complete oocyte development in vitro is described and discussed. (C) 1998 by Elsevier Science Inc.