Increased frequency and cell death of CD16+ monocytes with Mycobacterium tuberculosis infection
Increased frequency and cell death of CD16+ monocytes with Mycobacterium tuberculosis infection
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DOI：
10.1016/j.tube.2011.04.002
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发表时间：
2011-09-01
期刊：
影响因子：
3.2
通讯作者：
Rojas, Mauricio
中科院分区：
文献类型：
--
作者：
Castano, Diana;Garcia, Luis F.;Rojas, Mauricio
与健康对照组相比，结核病患者的单核细胞表现出功能和表型改变。为了确定这些差异是否可以用单核细胞亚群的变化来解释，在结核病患者和健康对照者中评估了CD 14和CD 16的表达;此外，还发现了一些与单核吞噬细胞成熟、分化和功能相关的标志物，如CD1a、CD1c、CD11b、CD11c、CD13、CD33、CD36、CD40、CD64、CD68、CD80、CD83、检测单核细胞亚群中的CD86、HLA-DR、CCR2、CCR5和非特异性酯酶（NSE）。患者的循环CD14（Hi）CD16（+）和CD14（Lo）CD16（+）单核细胞百分比增加。CD14（Hi）CD16（+）和CD14（Lo）CD16（+）细胞中表达CD11b、CD36、CD64、CD68、CD80、CD86、CCR2和NSE的单核细胞百分比低于CD14（Hi）CD16（-）单核细胞。M.在感染后6h，结核感染的CD 16（+）单核细胞比CD 16（-）细胞产生更多的TNF-α和更少的IL-10。分离的CD 16（+）单核细胞在分化为巨噬细胞期间自发发生凋亡;与分化为单核细胞衍生的巨噬细胞（MDM）的CD 16（-）单核细胞形成对比，其细胞死亡诱导最小。此外，在感染活支原体的CD 16（+）亚群中，有更多的Annexin V和碘化丙啶阳性单核细胞。CD16（-）单核细胞在24 h内的表达明显高于结核杆菌。在本研究建立的培养条件下，单核细胞亚群不分化为树突状细胞。这些结果表明，结核病患者循环中CD16（+）单核细胞的频率增加，这些单核细胞更倾向于产生TNF-α并在对M.肺结核感染。(C)2011爱思唯尔有限公司版权所有。
Monocytes from tuberculosis patients exhibit functional and phenotypical alterations compared with healthy controls. To determine whether these discrepancies can be explained by changes in monocyte subsets, the expression of CD14 and CD16 was evaluated in tuberculosis patients and healthy controls; additionally, some markers related to the mononuclear phagocytes maturation, differentiation and function, such as CD1a, CD1c, CD11b, CD11c, CD13, CD33, CD36, CD40, CD64, CD68, CD80, CD83, CD86, HLA-DR, CCR2, CCR5, and non-specific esterases (NSE) were determined in monocyte subsets. Patients had increased percentage of circulating CD14(Hi)CD16(+) and CD14(Lo)CD16(+) monocytes. The percentage of monocytes expressing CD11b, CD36, CD64, CD68, CD80, CD86, CCR2 and NSE was lower in CD14(Hi)CD16(+) and CD14(Lo)CD16(+) cells than in CD14(Hi)CD16(-) monocytes. M. tuberculosis infected CD16(+) monocytes produced more TNF-alpha and less IL-10 than CD16(-) cells at 6 h post-infection. Isolated CD16(+) monocytes spontaneously underwent apoptosis during differentiation into macrophages; in contrast to CD16(-) monocytes that became differentiated into monocyte-derived macrophages (MDM) with a minimal induction of cell death. In addition, there were more Annexin V and propidium iodide positive monocytes in the CD16(+) subset infected with live M. tuberculosis at 24 h than CD16(-) monocytes. Under the culture conditions established for this study, the monocyte subsets did not differentiate into dendritic cells. These results show that tuberculosis patients have an augmented frequency of CD16(+) circulating monocytes which are more prone to produce TNF-a and to undergo cell death in response to M. tuberculosis infection. (C) 2011 Elsevier Ltd. All rights reserved.