Immune response of male baboons to testis-specific LDH-C4
Immune response of male baboons to testis-specific LDH-C4
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DOI：
10.1016/s0010-7824(01)00227-x
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发表时间：
2001-08-01
期刊：
影响因子：
2.9
通讯作者：
Doncel, GF
中科院分区：
文献类型：
--
作者：
Goldberg, E;VandeBerg, JL;Doncel, GF
用含有睾丸特异性乳酸脱氢酶（LDH-C-4） b细胞表位和破伤风毒素混杂t细胞表位的嵌合肽免疫了四只性成熟雄性狒狒。LDH-C4是睾丸特异性乳酸脱氢酶同工酶，该蛋白的抗体显著降低雌性非人灵长类动物的生育能力。动物在第0天免疫，并在第29、61和344天进行加强注射。在最初的6个月和最后一次加强后定期测定血清特异性抗体。在初次免疫后的第61、127和183天进行睾丸活检。在最后一次增强剂之前和之后三次评估精子带结合。目前的研究表明，LDH-C4的这个表位不会引起自身免疫性疾病，并且这些免疫的雄性精子的带结合能力降低。这些结果表明，一种基于抗精子抗体发展的安全男性免疫避孕药是可行的。(C) 2001爱思唯尔科学公司版权所有。
Four sexually mature male baboons (Papio sp.) were immunized with a chimeric peptide containing a B-cell epitope of the testis-specific lactate dehydrogenase (LDH-C-4) and a promiscuous T-cell epitope of tetanus toxin. LDH-C4 is the testis-specific isozyme of lactate dehydrogenase, and antibodies to this protein reduce fertility significantly in female nonhuman primates. Animals were immunized on Day 0 and received booster injections at Days 29, 61, and 344 after priming. Serum specific antibodies were determined at regular intervals during the initial 6 months and after the last booster. Testis biopsies were taken at Days 61, 127, and 183 after the primary immunization. Sperm-zona binding was assessed prior to and three times after the last booster. The present study demonstrated that this epitope of LDH-C4 did not cause autoimmune disease and that sperm from these immunized males had a diminished zona binding capacity. These results suggest that a safe male immunocontraceptive based on development of anti-sperm antibodies may be feasible. (C) 2001 Elsevier Science Inc. All rights reserved.