Identification of a putative candidate gene encoding 7-dehydrocholesterol reductase involved in brassinosteroids biosynthesis for compact plant architecture in Cucumber (Cucumis sativus L.)
Identification of a putative candidate gene encoding 7-dehydrocholesterol reductase involved in brassinosteroids biosynthesis for compact plant architecture in Cucumber (Cucumis sativus L.)
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DOI：
10.1007/s00122-021-03802-5
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发表时间：
2021-03
影响因子：
5.4
通讯作者：
Mengru Zhang;Mengfei Song;Feng Cheng;Zhige Yang;Marzieh Davoudi;Jinfeng Chen;Q. Lou
中科院分区：
文献类型：
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作者：
Mengru Zhang;Mengfei Song;Feng Cheng;Zhige Yang;Marzieh Davoudi;Jinfeng Chen;Q. Lou
通过本体分离分析测序结合遗传定位的策略，CsDWF5被确定为参与油菜素内酯生物合成的7脱氢胆固醇还原酶的候选基因。摘要矮化结构是作物育种的重要目标之一。油菜素内酯（BRs）的生物合成和信号转导对植物的生长发育有重要影响，包括植物的结构。在这里，我们从ems诱导的黄瓜群体中鉴定了一个紧凑植物结构（cpa）突变体。植株呈极矮型，节间和叶柄缩短，叶片暗沉皱折。遗传分析表明，该病是由一个单隐性基因引起的。通过本体分离分析测序结合遗传作图的策略，CsDWF5编码参与甾醇生物合成的7-脱氢胆固醇还原酶，被确定为cpa的候选基因。在csdwf5incpa的第6内含子第1个碱基的剪接位点发现一个单核苷酸突变（G→A）导致3-bp插入（TAG），进而导致移码突变，得到一个过早终止密码子。与野生型相比，csdwf5基因在该突变体不同组织中的表达均显著下调。外源BR处理可以部分恢复cpaco的表型。转录组分析鉴定出1096个基因在突变体和野生型之间表现出差异表达。KEGG富集分析表明，BR生物合成和植物-病原体互作途径中差异表达基因显著富集。这些结果为黄瓜矮化表型的分子机制提供了新的视角。
Key messageBy the strategy of bulked segregant analysis sequencing combined with genetic mapping,CsDWF5, which encodes 7 dehydrocholesterol reductase that involved in brassinosteroids biosynthesis, was identified as the candidate gene forcpa.AbstractDwarf architecture is one of the most important breeding goals in crops. The biosynthesis and signal transduction of brassinosteroids (BRs) have a great impact on plant growth and development including plant architecture. Here, we identified acompact plant architecture(cpa) mutant from an EMS-induced cucumber population.cpadisplayed the extremely dwarf phenotype with shortened internode and petiole, darkened and wrinkled leaf. Genetic analysis revealed thatcpawas caused by a single recessive gene. By the strategy of bulked segregant analysis sequencing combined with genetic mapping,CsDWF5, encoding a 7-dehydrocholesterol reductase that involved in sterol biosynthesis, was identified as the candidate gene forcpa. One single nucleotide mutation (G→A) in splicing site causing 3-bp insertion (TAG) was found in the first base of the sixth intron ofCsDWF5incpa, which furtherly resulted in the frameshift mutation and got a premature stop codon. The expression ofCsDWF5gene was significantly down regulated in different tissues of thecpamutant compared with that in wild type. The phenotype ofcpacould be partially recovered by exogenous BR treatment. Transcriptome analysis identified 1096 genes that exhibited differential expression between thecpamutant and wild type. KEGG enrichment analysis indicated that differentially expressed genes were significantly enriched in BR biosynthesis and plant–pathogen interaction pathways. These results provide perspectives on the molecular mechanisms underlying the dwarfing phenotype in cucumber.