A rapid bioassay for detecting saxitoxins using a Daphnia acute toxicity test
A rapid bioassay for detecting saxitoxins using a Daphnia acute toxicity test
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DOI：
10.1016/j.envpol.2010.03.007
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发表时间：
2010-06-01
影响因子：
8.9
通讯作者：
Azevedo, Sandra M. F. O.
中科院分区：
文献类型：
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作者：
Ferrao-Filho, Aloysio da S.;Soares, Maria Carolina S.;Azevedo, Sandra M. F. O.
设计了利用水蚤和微藻检测原水样中蓝藻神经毒素的生物检测方法。在一个富营养化的水库中，对Seston的浮游植物和氰毒素进行了15个月的分析。以50%接触者有效制动时间(ET50)为终点。在暴露于含有有毒蓝藻的湖水中的0.5-3h之间，观察到游泳活动的瘫痪，然后在放置在对照水中后的24h内，游泳活动几乎完全恢复。从水库中分离的一株产萨曲霉毒素的圆柱体精子菌的生物测定也观察到了同样的效果。回归分析表明，ET50与细胞密度、生物量和岩藻毒素含量之间存在显著的相关性，表明湖水中大型水蚤的瘫痪是由环斑扇贝体内发现的岩藻毒素引起的。水蚤生物测定法是一种检测天然样品中快速作用神经毒素的灵敏方法，与小鼠生物测定法相比具有重要的优势。(C)2010爱思唯尔有限公司。保留所有权利。
Bioassays using Daphnia pulex and Moina micrura were designed to detect cyanobacterial neurotoxins in raw water samples. Phytoplankton and cyanotoxins from seston were analyzed during 15 months in a eutrophic reservoir. Effective time to immobilize 50% of the exposed individuals (ET50) was adopted as the endpoint. Paralysis of swimming movements was observed between similar to 0.5-3 h of exposure to lake water containing toxic cyanobacteria, followed by an almost complete recovery of the swimming activity within 24 h after being placed in control water. The same effects were observed in bioassays with a saxitoxin-producer strain of Cylindrospermopsis raciborskii isolated from the reservoir. Regression analysis showed significant relationships between ET50 vs. cell density, biomass and saxitoxins content, suggesting that the paralysis of Daphnia in lake water samples was caused by saxitoxins found in C. raciborskii. Daphnia bioassay was found to be a sensitive method for detecting fast-acting neurotoxins in natural samples, with important advantages over mouse bioassays. (C) 2010 Elsevier Ltd. All rights reserved.