Flexible Platform for In Situ Impedimetric Detection and Bioelectric Effect Treatment of Escherichia Coli Biofilms
Flexible Platform for In Situ Impedimetric Detection and Bioelectric Effect Treatment of Escherichia Coli Biofilms
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DOI：
10.1109/tbme.2018.2872896
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发表时间：
2019-05-01
影响因子：
4.6
通讯作者：
Ghodssi, Reza
中科院分区：
文献类型：
--
作者：
Huiszoon, Ryan C.;Subramanian, Sowmya;Ghodssi, Reza
目标：本文报告了一个实时监测和处理三维生物医学设备表面生物膜形成的平台。方法：我们利用由聚酰亚胺基板上图案化的金叉指电极组成的灵活平台。该装置被集成到导尿管的内部，并在定制开发的流动系统中进行表征。通过 100 Hz 交流、50 mV 信号幅度的阻抗变化来监测生物膜生长。结果：24 小时内阻抗下降 30%，对应于大肠杆菌生物膜的形成。该平台还可以通过生物电效应去除生物膜；低浓度的抗生素与施加的交流电压信号相结合导致生物膜协同减少，导致阻抗增加 12%。通过结晶紫染色进行的生物量表征证实，阻抗检测结果与传感器上生物膜生物量的变化相关。我们还演示了与基于芯片的阻抗转换器的集成，以实现小型化并允许现场无线实施。使用阻抗转换器测量的 5% 阻抗下降对应于生物膜的生长，复制了使用恒电位仪测量的趋势。结论：该平台为多种脆弱环境中的生物膜感染管理提供了一种有前途的解决方案。意义：生物膜是微生物的主要生长模式，细菌细胞在水合表面上定植并导致反复感染。由于生物膜生长的环境难以接近，且其对抗菌药物的耐受性不断增强，因此医疗设备的识别和去除提出了挑战。
Goal: This paper reports a platform for real-time monitoring and treatment of biofilm formation on three-dimensional biomedical device surfaces. Methods: We utilize a flexible platform consisting of gold interdigitated electrodes patterned on a polyimide substrate. The device was integrated onto the interior of a urinary catheter and characterization was performed in a custom-developed flow system. Biofilm growth was monitored via impedance change at 100 Hz ac with a 50 mV signal amplitude. Results: A 30% impedance decrease over 24 h corresponded to Escherichia coli biofilm formation. The platform also enabled removal of the biofilm through the bioelectric effect; a lowconcentration of antibiotic combinedwith the applied ac voltage signal led to a synergistic reduction in biofilm resulting in a 12% increase in impedance. Biomass characterization via crystal violet staining confirmed that the impedance detection results correlate with changes in the amount of biofilm biomass on the sensor. We also demonstrated integration with a chip-based impedance converter to enable miniaturization and allow in situ wireless implementation. A 5% impedance decrease measured with the impedance converter corresponded to biofilm growth, replicating the trend measured with the potentiostat. Conclusion: This platform represents a promising solution for biofilm infection management in diverse vulnerable environments. Significance: Biofilms are the dominant mode of growth for microorganisms, where bacterial cells colonize hydrated surfaces and lead to recurring infections. Due to the inaccessible nature of the environments where biofilms grow and their increased tolerance of antimicrobials, identification, and removal on medical devices poses a challenge.