ALLELE AND LOCUS-SPECIFIC DIFFERENCES IN CELL-SURFACE EXPRESSION AND THE ASSOCIATION OF HLA CLASS-I HEAVY-CHAIN WITH BETA-2-MICROGLOBULIN - DIFFERENTIAL-EFFECTS OF INHIBITION OF GLYCOSYLATION ON CLASS-I SUBUNIT ASSOCIATION
ALLELE AND LOCUS-SPECIFIC DIFFERENCES IN CELL-SURFACE EXPRESSION AND THE ASSOCIATION OF HLA CLASS-I HEAVY-CHAIN WITH BETA-2-MICROGLOBULIN - DIFFERENTIAL-EFFECTS OF INHIBITION OF GLYCOSYLATION ON CLASS-I SUBUNIT ASSOCIATION
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DOI：
10.1002/eji.1830180522
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发表时间：
1988-05-01
影响因子：
5.4
通讯作者：
PLOEGH, HL
中科院分区：
文献类型：
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作者：
NEEFJES, JJ;PLOEGH, HL
检测了HLAI类抗原的组装，以及单个N-连接的糖链在这一过程中的作用。我们观察到人类白细胞抗原-b基因座产物的正确组装和表面表达需要N-连接的糖基化，尽管在人类白细胞抗原-A和B基因座的等位基因序列中发现了相当大的差异。我们的结论是，单个N-连接的多糖可以以主要方式促进重(H)链的构象，该重(H)链适合与轻链β2-微球蛋白相关联，并且在这方面，碳水化合物链的存在而不是类型是重要的。人I类H链与β2-微球蛋白的结合呈现双相动力学，其中最初的快速阶段之后是一个较长的时期，在此期间不能测量到进一步的结合。人类白细胞抗原-C-H链最初合成的数量与人类白细胞抗原-A和B-H链相似，但与β-2-微球蛋白结合效率低，导致细胞表面低表达。I类抗原的组装和成熟的各个阶段，包括从高尔基体到细胞表面的转移，都表现出特征性的等位基因变异。
The assembly of HLA class I antigens, and the contribution of the single N-linked glycan to this process were examined. We observed a requirement for N-linked glycosylation in the proper assembly and surface expression of HLA-b locus products in particular, although considerable variation was seen within the allelic series of the HLA-A and B loci. We conclude that the single N-linked glycan can contribute in a major way to that conformation of the heavy (H) chain which is competent to associated with the light chain .beta.2-microglobulin, and that the presence, rather than the type, of carbohydrate chain is important in this respect. The association of human class I H chains with .beta.2-microglobulin shows biphasic kinetics, where an initially rapid phase is followed by a prolonged period during which no further association can be measured. It appears that HLA-C H chains are initially synthesized in amounts similar to HLA-A and B H chains, but associate inefficiently with .beta.2-microglobulin, resulting in low expression of HLA-C at the cell surface. The individual stages of assembly and maturation of class I antigens including the transfer from Golgi to cell surface were found to display characteristic allelic variation.