DNA-Templated Magnetic Nanoparticle-Quantum Dot Polymers for Ultrasensitive Capture and Detection of Circulating Tumor Cells
DNA-Templated Magnetic Nanoparticle-Quantum Dot Polymers for Ultrasensitive Capture and Detection of Circulating Tumor Cells
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DOI：
10.1002/adfm.201707152
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发表时间：
2018-04-05
影响因子：
19
通讯作者：
Ma, Nan
中科院分区：
文献类型：
--
作者：
Li, Zhi;Wang, Ganglin;Ma, Nan
循环肿瘤细胞（CTC）计数和分析已成为癌症诊断和预后的重要平台。然而，一个巨大的挑战是以快速和高通量的方式从血液样品中有效地捕获具有高纯度的低丰度CTC，用于准确和灵敏的CTC检测。本文中，开发了一类新的DNA模板化磁性纳米颗粒-量子点（QD）-适体共聚物（MQAP），用于从人血液中快速磁性分离CTC，具有高捕获效率和接近80%的纯度。CTC的表型同时在单细胞水平上用QD光致发光（PL）分析。这些MQAP是通过杂交链反应构建的，以实现放大的磁响应，相对于背景细胞对靶细胞的非凡结合选择性，以及用于单细胞检测的超亮系综QD PL。MQAP不含非特异性结合，否则会损害靶细胞的捕获纯度。因此，血液样品中的稀有CTC的容易分离和计数可以在20分钟内以高灵敏度和准确性实现。
Circulating tumor cell (CTC) enumeration and analysis has emerged as an important platform for cancer diagnosis and prognosis. A great challenge, however, is to efficiently capture low abundant CTCs with high purity from blood samples in a rapid and high-throughput manner for accurate and sensitive CTC detection. Herein, a new class of DNA-templated magnetic nanoparticle-quantum dot (QD)-aptamer copolymers (MQAPs) is developed for rapid magnetic isolation of CTCs from human blood with high capture efficiency and purity approaching 80%. The phenotype of CTCs is simultaneously profiled with QD photoluminescence (PL) at single cell level. These MQAPs are constructed through hybridization chain reaction to achieve amplified magnetic response, extraordinary binding selectivity for target cells over background cells, and ultra bright ensemble QD PL for single cell detection. MQAPs are free from nonspecific binding that would otherwise compromise the capture purity of target cells. As a result, facile isolation and enumeration of rare CTCs in blood samples could be achieved in 20 min with high sensitivity and accuracy.