Mutations in the Atp1p and Atp3p subunits of yeast ATP synthase differentially affect respiration and fermentation in Saccharomyces cerevisiae
Mutations in the Atp1p and Atp3p subunits of yeast ATP synthase differentially affect respiration and fermentation in Saccharomyces cerevisiae
复制标题
DOI：
10.1007/s10863-007-9071-4
复制
发表时间：
2007-04-01
影响因子：
3
通讯作者：
Thorsness, Peter E.
中科院分区：
文献类型：
--
作者：
Francis, Brian R.;White, Karen H.;Thorsness, Peter E.
ATP 1 -111是缺乏线粒体DNA的ymel A的缓慢生长表型的抑制因子，是由于线粒体ATP合酶（酵母中的Atplp）的α-亚基的位置111处苯丙氨酸取代缬氨酸。ATPI-111的抑制活性需要线粒体ATP合酶的完整的β（Atp 2 p）和γ（Atp 3 p）亚基，但不需要定子柄亚基B（Atp 4 p）和OSCP（Atp 5 p）。ATPI-111和ATP 1和ATP 3中的其他类似抑制突变增加了缺乏线粒体DNA的野生型菌株的生长速率。这些抑制性突变降低了含有完整线粒体染色体的酵母在需要氧化磷酸化的培养基上的生长速率，但在可发酵培养基上生长时不会。在培养酵母的时间老化的测量表明，ATP 1和ATP 3抑制等位基因的菌株，含有线粒体DNA的寿命比同基因野生型菌株。相反，缺乏线粒体DNA并含有这些突变的酵母细胞的时间寿命比同基因野生型菌株短。与野生型相比，携带ATP 1 -111的菌株的孢子活力降低，尽管ATP 1 -111增强缺乏线粒体DNA的孢子的存活。
ATP1-111, a suppressor of the slow-growth phenotype of ymel A lacking mitochondrial DNA is due to the substitution of phenylalanine for valine at position 111 of the alpha-subunit of mitochondrial ATP synthase (Atplp in yeast). The suppressing activity of ATPI-111 requires intact beta (Atp2p) and gamma (Atp3p) subunits of mitochondrial ATP synthase, but not the stator stalk subunits b (Atp4p) and OSCP (Atp5p). ATPI-111 and other similarly suppressing mutations in ATP1 and ATP3 increase the growth rate of wild-type strains lacking mitochondrial DNA. These suppressing mutations decrease the growth rate of yeast containing an intact mitochondrial chromosome on media requiring oxidative phosphorylation, but not when grown on fermentable media. Measurement of chronological aging of yeast in culture reveals that ATP1 and ATP3 suppressor alleles in strains that contain mitochondrial DNA are longer lived than the isogenic wild-type strain. In contrast, the chronological life span of yeast cells lacking mitochondrial DNA and containing these mutations is shorter than that of the isogenic wild-type strain. Spore viability,of strains bearing ATP1-111 is reduced compared to wild type, although ATP1-111 enhances the survival of spores that lacked mitochondrial DNA.