The IsTat 1.3 VSG multigene family in Trypanosoma brucei: retention of the expression linked copy through multiple antigenic switches.
The IsTat 1.3 VSG multigene family in Trypanosoma brucei: retention of the expression linked copy through multiple antigenic switches.
复制标题
布氏锥虫中的 IsTat 1.3 VSG 多基因家族：通过多个抗原开关保留表达连锁副本。
DOI：
10.1093/nar/12.23.9051
复制
发表时间：
1984
影响因子：
14.9
通讯作者：
Stuart,K
中科院分区：
文献类型：
--
作者：
Allison,J;Rothwell,V;Newport,G;Agabian,N;Stuart,K
在T.布鲁氏菌3型变异表面糖蛋白（VSG）基因家族约有10个成员，其中一个在微小染色体上具有端粒定位。在表达3 VSG的抗原变体中出现的3 VSG基因的表达连锁拷贝（ELC）也具有端粒位置，但与小染色体3 VSG基因不同，其在5′端空白区上游具有限制性位点。该ELC在（i）表达端粒7 VSG ELC的后续变体和（ii）复发变体和衍生自变体抗原类型（VAT）3和7的原环形式中保留在相同的端粒上。7 ELC在5 '贫瘠区域上游有一个限制性图谱，该图谱与3 ELC的图谱不同，但相似。这些数据表明，3和7 ELC在表达时位于不同的端粒上。
In the IsTaR 1 serodeme ofT. bruceithe 3 variant surface glycoprotein (VSG) gene family contains about 10 members, one of which has a telomeric location on a minichromosome. The expression linked copy (ELC) of the 3 VSG gene which occurs in an antigenic variant expressing the 3 VSG, also has a telomeric location but unlike the minichromosomal 3 VSG gene has restriction sites upstream from the 5′ barren region. This ELC is retained on the same telomere in (i) a subsequent variant that expresses a telomeric 7 VSG ELC and in (ii) relapse variants and procyclic forms derived from variant antigenic types (VATs) 3 and 7. The 7 ELC has a restriction map upstream from the 5′ barren region that differs from, but is similar to, that of the 3 ELC. These data indicate that the 3 and 7 ELCs are on different telomeres when expressed.