Malonyl isoflavone glucosides are chiefly hydrolyzed and absorbed in the colon.
Malonyl isoflavone glucosides are chiefly hydrolyzed and absorbed in the colon.
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DOI：
10.1021/jf404378r
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发表时间：
2014-03
影响因子：
6.1
通讯作者：
Hiroko Yonemoto-Yano;M. Maebuchi;K. Fukui;S. Tsuzaki;K. Takamatsu;M. Uehara
中科院分区：
文献类型：
--
作者：
Hiroko Yonemoto-Yano;M. Maebuchi;K. Fukui;S. Tsuzaki;K. Takamatsu;M. Uehara
丙二酰葡糖苷是大豆下胚轴的水溶性衍生物。本研究比较了丙二酰葡糖苷和非丙二酰葡糖苷在大鼠体内的水解和吸收。在口服丙二酰葡糖苷或葡糖苷后，测量了血浆中的葡糖苷酮浓度。禁食后，切除十二指肠、空肠、回肠和结肠，并制备匀浆。测量每种肠匀浆对每种糖苷的水解程度。丙二酰葡糖苷类的大鼠血浆中大豆苷元（如大豆苷元和黄豆黄素）的水平高于丙二酰葡糖苷类。给予丙二酰葡糖苷的大鼠血浆中大豆苷元的曲线下面积也显著大于给予丙二酰葡糖苷的大鼠。使用Caco-2细胞的转运实验表明，丙二酰葡糖苷降解为糖苷配基是肠吸收所必需的。丙二酰葡糖苷仅在结肠中水解，而丙二酰葡糖苷的水解发生在空肠、回肠和结肠中。这些结果表明，丙二酰葡糖苷比非丙二酰葡糖苷更有效地吸收。此外，在结肠中丙二酰甘草苷元的有效吸收有助于血浆中的丙二酰甘草苷元水平的显著增加。
Malonyl isoflavone glucosides are water-soluble derivatives of soybean hypocotyls. This study compared the hydrolysis and absorption of malonyl isoflavone glucosides and nonmalonyl isoflavone glucosides in rats. Plasma concentrations of isoflavones were measured after oral administration of malonyl isoflavone glucosides or isoflavone glucosides. After fasting, the duodenum, jejunum, ileum, and colon were excised, and homogenates were prepared. The extent of hydrolysis of each glucoside by each intestinal homogenate was measured. Plasma levels of isoflavone aglycones, such as daidzein and glycitein, were higher in rats administered malonyl isoflavone glucosides than in those administered isoflavone glucosides. The area under the curve of daidzein in plasma of rats administered malonyl isoflavone glucosides was also significantly greater than that in those administered isoflavone glucosides. A transport experiment using Caco-2 cells suggested that degradation of malonyl glucosides to aglycones is necessary for intestinal absorption. Malonyl isoflavone glucosides were hydrolyzed only in the colon, whereas hydrolysis of isoflavone glucosides occurred in the jejunum, ileum, and colon. These results indicated more effective absorption of malonyl isoflavone glucosides than of nonmalonyl isoflavone glucosides. Moreover, effective absorption of malonyl isoflavone aglycones in the colon contributed to the significant increase in plasma isoflavone levels.