OP-1 application in bone allograft integration: preliminary results in sheep experimental surgery
OP-1 application in bone allograft integration: preliminary results in sheep experimental surgery
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DOI：
10.1016/s0020-1383(08)70017-2
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发表时间：
2008-09-01
影响因子：
2.5
通讯作者：
Giardino, R.
中科院分区：
文献类型：
--
作者：
Donati, D.;Di Bella, C.;Giardino, R.
大块同种异体骨移植经常用于骨科重建手术。然而，长期随访的失败率约为25%。目的：刺激同种异体移植物的融合。材料和方法：为了刺激同种异体移植物的骨重塑，我们将重组人成骨蛋白-1（rh-OP-1，也称为骨形态发生蛋白-7，BMP-7）应用于长骨临界尺寸缺陷羊模型。我们在 9 只羊中制造了 3 厘米的骨骨膜跖骨缺损，并用单独的结构同种异体移植物（对照组，4 只动物）或添加了 rh-BMP-7 的同种异体移植物（BMP 组，5 只动物）替代。进行了放射照相、力学、组织学和组织形态学分析。结果：术后前 8 周内，与对照组相比，BMP 组的 X 射线显示骨痂形成更好、更快。 16 周后，BMP 组出现了更高的骨重塑证据。在第 4、8 和 16 周时，BMP 组中连接部位的射线照相加热更为明显。螺钉拔除的机械测试显示两组之间没有统计学差异，组织形态计量学显示同种异体移植物内新形成的骨也没有差异。与对照组相比，BMP 组的移植物吸收率更高。 BMP 组中新形成血管的渗透率显着更高。结论：这些发现表明，将 BMP-7 添加到同种异体结构骨移植物中，通过刺激新血管生成和成骨活性诱导移植物的早期重塑，且对机械强度和临床结果没有负面影响。 (C) 2008 Elsevier Ltd. 保留所有权利。
Massive bone allografts are frequently used in orthopaedic reconstructive surgery. However the failure rate at long term follow-up is around 25%.Aim: Stimulation of allograft incorporation.Materials and Methods: In order to stimulate bone remodeling of an allograft we applied recombinant human osteogenic protein-1 (rh-OP-1, also know as bone morphogenetic protein-7, BMP-7) to a long bone critical size defect sheep model. In nine sheep we created a 3 cm osteoperiosteal metatarsal defect replaced with a structural allograft alone (control group, 4 animals), or an allograft added with rh-BMP-7 (BMP group, 5 animals). Radiographic, mechanical, histological and histomorphometric analysis were performed.Results: X-rays in the BMP group showed a better and faster callus formation, compared to the control group within the first 8 weeks after surgery. After 16 weeks there was a higher evidence of bone remodeling in the BMP group. Radiographic heating at junction sites was more evident in the BMP group at 4, 8 and 16 weeks. Mechanical testing on screw extraction showed no statistical differences between the two groups and histomorphometry showed no difference in terms of newly formed bone inside the allograft as well. The resorption rate of the graft was higher in the BMP group in comparison to the control group. The penetration of newly formed vessels was significantly higher in the BMP group.Conclusions: These findings indicate that BMP-7 added to a structural bone allograft inducing early remodeling of the graft through stimulation of neo-angiogenesis and osteociastic activity, without negative effects in mechanical strength and clinical outcome. (C) 2008 Elsevier Ltd. All rights reserved.