VOLUME REGULATION IN COCHLEAR OUTER HAIR-CELLS
VOLUME REGULATION IN COCHLEAR OUTER HAIR-CELLS
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DOI：
10.1016/0378-5955(93)90107-c
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发表时间：
1993-09-01
期刊：
影响因子：
2.8
通讯作者：
BOBBIN, RP
中科院分区：
文献类型：
--
作者：
CRIST, JR;FALLON, M;BOBBIN, RP
许多置于低张介质中的细胞最初肿胀，然后迅速经历调节性体积减小(RVD)以恢复到原始体积。再次暴露在等渗溶液中会导致细胞收缩，随后调节性体积增加(RVI)。以前的研究表明，将分离的外毛细胞放在低渗介质中会膨胀并保持这种形状，直到回到原来的介质中。我们重新检查了这种明显缺乏细胞体积调节的毛细胞。分离豚鼠耳蜗单核细胞，机械分离分散，置于Hank‘s平衡盐溶液(HBS)中。在所研究的细胞中，将灌流液切换到低张HBS(290-280 mmol/kg)15分钟会导致内毛细胞立即缩短(即体积增加)。在26%的细胞中，在低张介质(RVD)灌流期间，随着细胞长度的增加，细胞长度恢复到原始长度。在显示RVD的细胞中，12%的细胞也显示了RVI。去掉胶原酶和增加钙离子浓度不会增加RVD细胞的百分比，而Gd3+(Gd3+，10微米)则将这一百分比降低到零。这是分离的毛细胞进行细胞体积调节的第一个报告。
Many cells placed in a hypotonic medium initially swell and then rapidly undergo a regulatory volume decrease (RVD) to return towards original volume. Re-exposure to the isotonic solution results in the cells shrinking followed by a regulatory volume increase (RVI). Previous studies have shown that isolated outer hair cells (OHCs) placed in a hypotonic medium swell and maintain this shape until returned to the original medium. We re-examined this apparent lack of cell volume regulation in OHCs. OHCs were isolated from guinea pig cochleae, mechanically dissociated and dispersed, and placed in a Hank's balanced salt solution (HBS). In the cells studied, switching the perfusate to a hypotonic HBS (290-280 mmol/kg) for 15 min resulted in an immediate shortening of the OHCs (i.e., volume increase). In 26% of the cells, this increase was followed by a return to original length during the time the cell was perfused with the hypotonic medium, a RVD. Twelve percent of the cells demonstrating a RVD also displayed a RVI. Omitting collagenase and increasing Ca2+ concentration did not increase the percentage of cells displaying a RVD, while gadolinium (Gd3+, 10 muM) decreased the percentage to zero. This is the first report of isolated OHCs undergoing cell volume regulation.