Typing of methicillin-resistant Staphylococcus aureus in a university hospital setting by using novel software for spa repeat determination and database management
Typing of methicillin-resistant Staphylococcus aureus in a university hospital setting by using novel software for spa repeat determination and database management
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DOI：
10.1128/jcm.41.12.5442-5448.2003
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发表时间：
2003-12-01
影响因子：
9.4
通讯作者：
Vogel, U
中科院分区：
文献类型：
--
作者：
Harmsen, D;Claus, H;Vogel, U
金黄色葡萄球菌的spa基因编码蛋白A，用于耐甲氧西林金黄色葡萄球菌（MRSA）的分型。我们利用spa基因重复区序列分型研究了德国某大学医院的MRSA流行病学。107株和84株分别在10个月和4个月的时间内进行了研究。重复序列和spa类型由Ridom StaphType测定，Ridom StaphType是一种新型的软件工具，可以快速测定重复序列，管理和检索数据，并在DNA序列色谱自动质量控制后基于互联网分配新的spa类型。对最丰富的spa类型的代表性分离物进行了多位点序列分型和脉冲场凝胶电泳。在第二个研究期间，两种主要spa类型中的一种被一种克隆相关变体所取代。10种独特的温泉类型，在两个研究时期均匀分布，被恢复。数据显示，在大学医院设置克隆循环的快速动态。Spa分型对流行分离株的追踪有一定的价值。数据显示，在MRSA高发科室进行spa分型的主要目的之一是反驳流行病学上认为的MRSA传播。
The spa gene of Staphylococcus aureus encodes protein A and is used for typing of methicillin-resistant Staphylococcus aureus (MRSA). We used sequence typing of the spa gene repeat region to study the epidemiology of MRSA at a German university hospital. One hundred seven and 84 strains were studied during two periods of 10 and 4 months, respectively. Repeats and spa types were determined by Ridom StaphType, a novel software tool allowing rapid repeat determination, data management and retrieval, and Internet-based assignment of new spa types following automatic quality control of DNA sequence chromatograms. Isolates representative of the most abundant spa types were subjected to multilocus sequence typing and pulsed-field gel electrophoresis. One of two predominant spa types was replaced by a clonally related variant in the second study period. Ten unique spa types, which were equally distributed in both study periods, were recovered. The data show a rapid dynamics of clone circulation in a university hospital setting. spa typing was valuable for tracking of epidemic isolates. The data show that disproval of epidemiologically suggested transmissions of MRSA is one of the main objectives of spa typing in departments with a high incidence of MRSA.