Real-time nanodiamond thermometry probing in vivo thermogenic responses
Real-time nanodiamond thermometry probing in vivo thermogenic responses
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DOI：
10.1126/sciadv.aba9636
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发表时间：
2020-09-01
期刊：
影响因子：
13.6
通讯作者：
Kage-Nakadai, Eriko
中科院分区：
文献类型：
--
作者：
Fujiwara, Masazumi;Sun, Simo;Kage-Nakadai, Eriko
生物体内的实时温度监测提供了对其生物活动的直接测量。然而，这是具有挑战性的生物相容性的温度计的尺寸减小到亚微米，尽管它们的潜在应用的热成像的亚组织结构与单细胞分辨率。在这里，使用量子纳米温度计的基础上光学可访问的电子自旋纳米金刚石，我们证明了在体内实时温度监测内秀丽隐杆线虫蠕虫。我们开发了一种显微镜系统，该系统集成了快速对接样品室、颗粒跟踪和误差校正过滤器，用于监测活蠕虫内移动的纳米金刚石的温度，精度为+/- 0.22摄氏度。利用这个系统，我们根据蠕虫在线粒体解偶联剂的化学刺激期间的产热反应来确定温度的升高。我们的技术表明，在活体动物的温度信息的亚微米本地化和直接识别其药理产热，这可能允许量化的基础上，温度的生物活性。
Real-time temperature monitoring inside living organisms provides a direct measure of their biological activities. However, it is challenging to reduce the size of biocompatible thermometers down to submicrometers, despite their potential applications for the thermal imaging of subtissue structures with single-cell resolution. Here, using quantum nanothermometers based on optically accessible electron spins in nanodiamonds, we demonstrate in vivo real-time temperature monitoring inside Caenorhabditis elegans worms. We developed a microscope system that integrates a quick-docking sample chamber, particle tracking, and an error correction filter for temperature monitoring of mobile nanodiamonds inside live adult worms with a precision of +/- 0.22 degrees C. With this system, we determined temperature increases based on the worms' thermogenic responses during the chemical stimuli of mitochondrial uncouplers. Our technique demonstrates the submicrometer localization of temperature information in living animals and direct identification of their pharmacological thermogenesis, which may allow for quantification of their biological activities based on temperature.