The preventive effects of inulin, cellulose, and their mixture on colorectal cancer liver metastasis in mice by regulating gut microbiota.
The preventive effects of inulin, cellulose, and their mixture on colorectal cancer liver metastasis in mice by regulating gut microbiota.
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DOI：
10.1111/1750-3841.16772
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发表时间：
2023-10
影响因子：
3.9
通讯作者：
Chuhui Wang;Tongtong Lan;Zhao Chen;Xiaowen Wang;Yisa Han;Ning Yang;Zhen Xu;Hui Li;Meng Tao;Yang Song
中科院分区：
文献类型：
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作者：
Chuhui Wang;Tongtong Lan;Zhao Chen;Xiaowen Wang;Yisa Han;Ning Yang;Zhen Xu;Hui Li;Meng Tao;Yang Song
许多研究发现，膳食纤维可以预防结直肠癌（CRC）。由于转移，CRC患者的存活率显著降低。然而，关于膳食纤维对CRC转移的影响知之甚少。在这项研究中，我们分析了菊粉，纤维素，以及它们的混合物在小鼠原位移植模型中对结直肠癌转移的影响。将BALB/C雄性小鼠分为正常对照（NC）（AIN-93 M饲料）、MOD（AIN-93 M饲料）、INU（10%w/w菊糖）、CEL（10%w/w纤维素）和MIX（5%w/w菊糖+5%w/w纤维素）组。膳食纤维摄入量可抑制荷瘤小鼠的原位瘤重、肝重和肝转移面积（p < 0.05），提高荷瘤小鼠的生存率。与NC组相比，MOD组β-catenin和上皮标志物E-cadherin的表达降低，而间质标志物N-cadherin、MMP-9和VEGF的表达升高。所有菊粉、纤维素及其混合物都恢复了肠道微生物群的多样性，并且它们分别增加了双歧杆菌目、乳酸杆菌和毛螺菌科的相对丰度。菊粉恢复了乙酸、丙酸、异丁酸和丁酸的水平。斯皮尔曼相关性分析结果显示，5种属与6种短链脂肪酸（SCFA）之间存在正相关性（校正后p < 0.05）。总之，所有菊粉、纤维素及其混合物对CRC转移具有抑制作用，这可以通过调节肠道微生物群、SCFA的产生以及抑制上皮向间充质转化过程来实现。在三个膳食纤维干预组中，菊粉的抑制作用更为显著。
Many studies have found that dietary fiber can protect against colorectal cancer (CRC). Survival in CRC patients is significantly reduced due to metastasis. However, little is known regarding the impact of dietary fiber on the CRC metastasis. In this study, we analyzed the effects of inulin, cellulose, and their mixture on CRC metastasis in a murine orthotopic transplantation model. BALB/C male mice were divided into the normal control (NC) (AIN-93 M diet), MOD (AIN-93 M diet), INU (10% w/w inulin), CEL (10% w/w cellulose), and MIX (5% w/w inulin + 5% w/w cellulose) groups. Dietary fiber intake inhibited the weights of the orthotopic tumors, liver weights, and liver metastasis area (p < 0.05) and improved the survival rate of tumor-bearing mice. Compared to the NC, the expression of β-catenin and the epithelial marker E-cadherin were lower, and that of mesenchymal markers, such as N-cadherin, MMP-9, and VEGF, were higher in the MOD group. All inulin, cellulose, and their mixture restored the gut microbiota diversity, and they, respectively, increased the relative abundance of Bifidobacteriales, Lactobacillus, and Lachnospiraceae. Inulin restored the levels of acetic acid, propionic acid, isobutyric acid, and butyric acid. Spearman correlation analysis results showed that there was a positive correlation between five genera and six short-chain fatty acids (SCFAs) (adjusted p < 0.05). In conclusion, all inulin, cellulose, and their mixture have inhibitory effects on CRC metastasis, which may be achieved by the regulation of gut microbiota, the production of SCFAs, and the inhibition of the epithelial-to-mesenchymal transition process. Among the three dietary fiber intervention groups, the inhibitory effect of inulin is more significant.