Integrin expression is upregulated during early healing in a canine intrasynovial flexor tendon repair and controlled passive motion model
Integrin expression is upregulated during early healing in a canine intrasynovial flexor tendon repair and controlled passive motion model
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DOI：
10.3109/03008209809021505
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发表时间：
1998-01-01
影响因子：
2.9
通讯作者：
Amiel, D
中科院分区：
文献类型：
--
作者：
Harwood, FL;Monosov, AZ;Amiel, D
To explore crucial early molecular events involved in contact healing of the intrasynovial flexor tendon, integrin expression was evaluated at the transcriptional and post-transcriptional levels during the first two weeks following injury, repair and controlled passive motion in a canine model, Specifically, immunohistochemical and reverse transcription polymerase chain reaction (RT-PCR) techniques were employed to evaluate expression of the fibronectin, vitronectin and endothelial cell binding integrin receptor subunits alpha 5, alpha v and alpha 6, along,vith the common beta 1 subunit, The two techniques revealed increasing expression of the four subunits over the two week post-repair period. Immunohistochemistry revealed that beta 1 and alpha 5 expression was concentrated in the epitenon layer near the repair site and interiorly within the wound area, while alpha 6 was associated with capillary-forming endothelial cells near the wound. RT-PCR and quantitation by NM image analysis demonstrated peak messenger RNA expression of beta 1 and alpha 5 at ten days post-repair and peak expression of alpha 6 and alpha v at 15 days. The results in this study correlate well with previous results demonstrating increased fibronectin deposition and angiogenesis during the same time period in a similar injury/repair model.
