Inflammatory gene signature in ulcerative colitis with cDNA macroarray analysis
Inflammatory gene signature in ulcerative colitis with cDNA macroarray analysis
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DOI：
10.1111/j.1365-2036.2005.02443.x
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发表时间：
2005-05-01
影响因子：
7.6
通讯作者：
Imai, K
中科院分区：
文献类型：
--
作者：
Okahara, S;Arimura, Y;Imai, K
背景：大多数溃疡性结肠炎的芯片分析都集中于识别溃疡性结肠炎的易感基因。目的：利用cDNA微阵列阐明溃疡性结肠炎结肠粘膜炎症过程中基因表达的变化。方法：通过结肠镜活检获得23例溃疡性结肠炎患者中，炎症和非炎症标本各16份（总共32份样本用于个体分析）。用于成对分析的 32 个样本中的 18 个由 9 个样本对组成，每对样本来自同一患者。我们使用 cDNA 宏阵列检查了大约 1300 个基因的表达谱。在两种分析中使用两种统计方法、t检验和微阵列显着性分析进行比较。实时聚合酶链式反应证实了宏阵列分析中重要基因的重现性。结果：我们在发炎粘膜中检测到 5 个上调基因，分为促炎基因（MRP14、GRO gamma 和 SAA1）和抗炎基因（TIMP1 和 Elafin），以及在非发炎粘膜中检测到 1 个上调基因（L-FABP）。结论：由于本研究中的 cDNA 宏阵列分析准确反映了基因表达的总体概况。在溃疡性结肠炎的临床环境中，所鉴定的基因将直接适用于活动性溃疡性结肠炎的诊断或作为新的治疗靶点。
Background: Most array analyses of ulcerative colitis have focused on identifying susceptibility genes for ulcerative colitis.Aim: To clarify the changes in gene expression during inflammation in ulcerative colitis colon mucosa using cDNA macroarray.Methods: From 23 ulcerative colitis patients, 16 each of inflamed and non-inflamed specimens (total 32 samples for individual analysis) were obtained by colonoscopic biopsy. Eighteen of the 32 samples, used for pairwise analysis, consisted of nine sample pairs, each pair being from the same patient. We examined expression profiles of approximately 1300 genes with cDNA macroarray. Comparisons were made using two kinds of statistics, t-test and significance analysis of microarray in both analyses. The reproducibility of significant genes from the macroarray analysis was confirmed by real-time ploymerase chain reaction.Results: We detected five upregulated genes, categorized into proinflammatory genes (MRP14, GRO gamma and SAA1) and anti-inflammatory genes (TIMP1 and Elafin) in inflamed mucosa, and one upregulated gene (L-FABP) in non-inflamed mucosa.Conclusions: As the cDNA macroarray analysis in this study exactly reflects the total profile of gene expression in the clinical setting of ulcerative colitis, the genes identified will be directly applicable to diagnostics or as novel therapeutic targets in active ulcerative colitis.