Functional analysis of bipartite begomovirus coat protein promoter sequences
Functional analysis of bipartite begomovirus coat protein promoter sequences
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DOI：
10.1016/j.virol.2008.03.012
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发表时间：
2008-06-20
期刊：
影响因子：
3.7
通讯作者：
Sunter, Garry
中科院分区：
文献类型：
--
作者：
Lacatus, Gabriela;Sunter, Garry
我们证明，AL 2基因的甘蓝曲叶病毒（CaLCuV）激活CP启动子在叶肉中，并采取行动去抑制启动子在维管组织中，类似于观察到的番茄金色花叶病毒（TGMV）。结合研究表明，序列介导的抑制和激活的TGMV和CaLCuV CP启动子特异性结合不同的核因子常见的本塞姆氏烟草，菠菜和番茄。然而，染色质免疫沉淀表明，TGMV AL 2可以与这两个序列独立地相互作用。来自不同作物物种的核蛋白与二分型和单分型菜豆花叶病毒（包括TGMV、CaLCuV、辣椒金色花叶病毒和番茄黄化曲叶病毒）中保守的病毒序列的结合表明，二分型菜豆花叶病毒结合共同的宿主因子来调节CP启动子。这与其中AL2与细胞转录机制的不同组分相互作用的模型一致，所述细胞转录机制结合对于抑制和激活菜豆花叶病毒CP启动子重要的病毒序列。(c)2008年爱思唯尔公司All rights reserved.
We demonstrate that the AL2 gene of Cabbage leaf curl virus (CaLCuV) activates the CP promoter in mesophyll and acts to derepress the promoter in vascular tissue, similar to that observed for Tomato golden mosaic virus (TGMV). Binding studies indicate that sequences mediating repression and activation of the TGMV and CaLCuV CP promoter specifically bind different nuclear factors common to Nicotiana benthamiana, spinach and tomato. However, chromatin immunoprecipitation demonstrates that TGMV AL2 can interact with both sequences independently. Binding of nuclear protein(s) from different crop species to viral sequences conserved in both bipartite and monopartite begomoviruses, including TGMV, CaLCuV, Pepper golden mosaic virus and Tomato yellow leaf curl virus suggests that bipartite begomoviruses bind common host factors to regulate the CP promoter. This is consistent with a model in which AL2 interacts with different components of the cellular transcription machinery that bind viral sequences important for repression and activation of begomovirus CP promoters. (c) 2008 Elsevier Inc. All rights reserved.