Sequential preparation of rat liver microsomal and Golgi membranes.
Sequential preparation of rat liver microsomal and Golgi membranes.
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大鼠肝微粒体和高尔基体膜的连续制备。
DOI：
10.1016/0005-2736(78)90163-3
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发表时间：
1978
期刊：
影响因子：
--
通讯作者：
Lennart Eriksson
中科院分区：
文献类型：
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作者：
Göran Andersson;Göran Andersson;U. Torndal;Lennart Eriksson;Lennart Eriksson
建立了从同一块大鼠肝脏中制备微粒体、微粒体亚组分和高尔基膜的新方法。可用于制备的最小量肝脏约为1g（湿重）。内质网标记酶总活性的35%在微粒体部分恢复。该回收率与我们实验室使用其他程序获得的回收率大致相同。根据标记酶计算，高尔基体膜、线粒体、溶酶体和质膜占微粒体蛋白的不到13%。高尔基体膜的制备必须分两步进行，以达到合理的回收率，因此具有代表性的样品既包含极低密度的富含脂蛋白的高尔基体囊泡，也包含较重的贮池元素。从酒精处理过的动物肝脏中恢复的高尔基体部分中udp -半乳糖转移酶活性约为总颗粒部分中总活性的30%。
A new procedure for the preparation of microsomes, microsomal subfractions and Golgi membranes from the same piece of rat liver has been developed. The smallest amount of liver with which the preparation can be performed is about 1 g (wet weight).35% of the total activity of marker enzymes for the endoplasmic reticulum was recovered in the microsomal fraction. This recovery is approximately the same as that obtained in our laboratories using other procedures. Golgi membranes, mitochondria, lysosomes and plasma membranes represent less than 13% of the microsomal protein as calculated on the basis of marker enzymes.Golgi membranes must be prepared in two steps to achieve a reasonable recovery and thus a representative sample containing both very low density lipoprotein-rich Golgi vesicles and the heavier cisternal elements. The recovery of UDP-galactosyltransferase activity in the Golgi fraction from the livers of alcohol-treated animals is around 30% of the total activity in the total particulate fraction.