Identification of an Embryonic Cell-Specific Region within the Pineapple SERK1 Promoter
Identification of an Embryonic Cell-Specific Region within the Pineapple SERK1 Promoter
复制标题
菠萝 SERK1 启动子内胚胎细胞特异性区域的鉴定
DOI：
10.3390/genes10110883
复制
发表时间：
2019-11-01
期刊：
影响因子：
3.5
通讯作者：
He, Junhu
中科院分区：
文献类型：
--
作者：
Luan, Aiping;He, Yehua;He, Junhu
植物组织培养方法，如体细胞胚胎发生，是传统植物繁殖育种方法的有吸引力的替代方法。然而，它们往往效率有限。体细胞胚胎发生受体激酶（SERK）1是菠萝等多种植物早期体细胞胚胎发生的标记基因。它可以被选择性地诱导并促进体细胞胚胎发生的关键步骤。我们通过构建一系列携带GUS（β-葡萄糖醛酸酶）报告基因的载体，研究了AcSERK 1的胚胎细胞特异性转录调控。将这些载体转染到胚性和非胚性愈伤组织中，并分析了三个未成熟胚阶段和启动子片段的胚特异性活性。我们发现，AcSERK 1的调控序列缺乏-983 nt ~-880 nt，其中包括转录起始位点，在菠萝胚性愈伤组织中的活性显着降低，伴随着胚胎细胞特异性启动子活性的丧失。因此，该片段是胚胎细胞具有高度特异性启动子活性的必需功能片段，并且它仅在体细胞胚发育的早期阶段至球形胚阶段具有活性。本研究为鉴定提高菠萝和其他植物体细胞胚胎发生效率的机制奠定了基础。
Plant tissue culture methods, such as somatic embryogenesis, are attractive alternatives to traditional breeding methods for plant propagation. However, they often suffer from limited efficiency. Somatic embryogenesis receptor kinase (SERK)1 is a marker gene of early somatic embryogenesis in several plants, including pineapple. It can be selectively induced and promotes a key step in somatic embryogenesis. We investigated the embryonic cell-specific transcriptional regulation of AcSERK1 by constructing a series of vectors carrying the GUS (Beta-glucuronidase) reporter gene under the control of different candidate cis-regulatory sequences. These vectors were transfected into both embryonic and non-embryonic callus, and three immature embryo stages and the embryonic-specific activity of the promoter fragments was analyzed. We found that the activity of the regulatory sequence of AcSERK1 lacking −983 nt ~−880 nt, which included the transcription initiation site, was significantly reduced in the embryonic callus of pineapple, accompanied by the loss of embryonic cell-specific promoter activity. Thus, this fragment is an essential functional segment with highly specific promoter activity for embryonic cells, and it is active only from the early stages of somatic embryo development to the globular embryo stage. This study lays the foundation for identifying mechanisms that enhance the efficiency of somatic embryogenesis in pineapple and other plants.