Embedding Functional Biomacromolecules within Peptide-Directed Metal-Organic Framework (MOF) Nanoarchitectures Enables Activity Enhancement
Embedding Functional Biomacromolecules within Peptide-Directed Metal-Organic Framework (MOF) Nanoarchitectures Enables Activity Enhancement
复制标题
将功能性生物大分子嵌入肽导向的金属有机框架（MOF）纳米结构中可增强活性
DOI：
10.1002/anie.202005529
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发表时间：
2020-06-08
影响因子：
16.6
通讯作者：
Ouyang, Gangfeng
中科院分区：
文献类型：
--
作者：
Chen, Guosheng;Huang, Siming;Ouyang, Gangfeng
合理地定制一个坚固的人工涂层可以提高脆弱的生物大分子的寿命。然而，由于基底可及性的降低，涂层也可以抑制客体的活性。在此，我们报告了一种肽导向的策略，使在原位剪裁的MOF-笼罩的生物杂合成可控的纳米结构。通过肽调节剂，可以将MOF生物杂交体从不同的3D微孔结构塑造成2D中孔层。使用这种温和的策略，我们表明，MOF涂层的纳米结构显着影响所包含的生物大分子的生物功能。包埋在新型2D中孔纺锤形MOFs（2D MSMOFs）中的生物大分子与迄今为止探索的3D微孔MOFs相比具有显着增加的生物活性。这种改善是由于二维MSMOFs中扩散路径缩短和孔道扩大所致。同时，薄的2D MSMOF层还可以通过结构限制对宿主生物大分子或蛋白质支架生物矿物提供优异的保护。
Rationally tailoring a robust artificial coating can enhance the life-time of fragile biomacromolecules. However, the coating also can restrain the activity of the guest because of the decreased substrate accessibility. Herein, we report a peptide-directed strategy that enables in situ tailoring of the MOF-shrouded biohybrids into controllable nanoarchitectures. The MOF biohybrid can be shaped from different 3D microporous architectures into a 2D mesoporous layer by a peptide modulator. Using this mild strategy, we show that the nanoarchitectures of the MOF coatings significantly affect the biological functions of the contained biomacromolecules. The biomacromolecules entrapped within the novel 2D mesoporous spindle-shaped MOFs (2D MSMOFs) have significantly increased bioactivity compared to when encased within the hitherto explored 3D microporous MOFs. The improvement results from the shortened diffusion path and enlarged pore channel in 2D MSMOFs. Meanwhile, the thin 2D MSMOF layer also can provide excellent protection of the hosted biomacromolecules or protein-scaffolded biominerals through structural confinement.