A PUTATIVE 2ND-CELL-DERIVED ONCOGENE OF THE AVIAN LEUKEMIA RETROVIRUS-E26
A PUTATIVE 2ND-CELL-DERIVED ONCOGENE OF THE AVIAN LEUKEMIA RETROVIRUS-E26
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DOI：
10.1038/306395a0
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发表时间：
1983-01-01
期刊：
影响因子：
64.8
通讯作者：
STEHELIN, D
中科院分区：
文献类型：
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作者：
LEPRINCE, D;GEGONNE, A;STEHELIN, D
急性禽白血病逆转录病毒AMV和E26均在体内诱导成髓细胞形成，并在体外转化成髓细胞1 - 5。这两种病毒都含有最初描述为AMV6的致癌基因v- myb7。与AMV不同，E26在体内具有诱导成红细胞形成和转化成红细胞的额外能力4,5。先前的分析表明，E26的基因组也含有与病毒复制基因6,8,9无关的v-myband不同的核苷酸序列。利用分子克隆的E26原病毒，我们现在已经确定了位于v-myb旁边的一个新的核苷酸序列，命名为v-ets（fore26特异性）10 ~ 1.5千碱基对（kbp）。v-ets具有推定的新致癌基因的所有结构特征：它有一个保守的细胞对应物c-ets，在一些正常的鸡细胞中转录为一个主要的7.5 kb的聚腺苷化RNA。虽然我们的研究结果还有待于进一步阐明其生物学意义，但我们认为，v- etv可能是一个新的致癌基因，可以解释E26的额外转化特性，或者增强v-myboncogene的转化特性。
The acute avian leukaemia retroviruses AMV and E26 both induce myeloblastosisin vivoand transform myeloblastsin vitro1–5. Both viruses contain the oncogene v-mybfirst described for AMV6,7. Unlike AMV, E26 has the additional capacity to induce erythroblastosisin vivoand to transform erythroblasts4,5. Previous analyses indicated that the genome of E26 also contained nucleotide sequences distinct from v-myband unrelated to viral replicative genes6,8,9. Using a molecularly cloned E26 provirus, we have now identified a novel nucleotide sequence designated v-ets(forE-twenty-six specific)10of ∼1.5 kilobase pairs (kbp) located next to v-myb. v-etspossesses all the structural characteristics of a putative new oncogene: it has a conserved cellular counterpart c-etswhich is transcribed in some normal chicken cells as a major 7.5-kb polyadenylated RNA. Although our results now await elucidation of their biological significance, we propose that v-etscould be a new oncogene accounting for the additional transforming properties of E26, or potentiating the transforming properties of the v-myboncogene.