STUDY OF MULTIPLE FIBRILLARIN MESSENGER-RNAS REVEALS THAT 3' END FORMATION IN SCHIZOSACCHAROMYCES-POMBE IS SENSITIVE TO COLD SHOCK
STUDY OF MULTIPLE FIBRILLARIN MESSENGER-RNAS REVEALS THAT 3' END FORMATION IN SCHIZOSACCHAROMYCES-POMBE IS SENSITIVE TO COLD SHOCK
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DOI：
10.1093/nar/21.8.1881
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发表时间：
1993-04-25
影响因子：
14.9
通讯作者：
LAPEYRE, B
中科院分区：
文献类型：
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作者：
GIRARD, JP;FELIU, J;LAPEYRE, B
原纤蛋白是一种与小核仁RNA相关的核仁蛋白，并且是前rRNA加工所需的。我们已经克隆并鉴定了裂殖酵母裂殖酵母中编码原纤蛋白的基因，并在各种条件下进行了表达。裂殖酵母原纤蛋白是一种由305个氨基酸组成的蛋白质，在整个进化过程中高度保守。在非洲爪蟾、人或酿酒酵母中，检测到单个原纤蛋白mRNA，而在粟酒裂殖酵母中，单拷贝基因编码在3'端不同的不同mRNA。在正常生长条件下，检测到1.1和1.35 kb的两种mRNA，其中1.1 kb的丰度最高。这两种mRNA的总量和相对丰度都受到低温暴露的强烈影响，即1.1kb的mRNA几乎消失，而1.35kb的mRNA减少不太明显。一个3.2kb的新物种在细胞中积累，其中包含一个异常长的2kb的3'非翻译区。我们已经发现，将细胞暴露于冷休克对粟酒裂殖酵母中的3'末端形成具有深远的影响，因为几种其他mRNA的转录也能够跳过正常的3'末端位点以终止于更下游的位点。
Fibrillarin is a nucleolar protein which is associated with small nucleolar RNAs, and is required for pre-rRNA processing. We have cloned and characterized the gene encoding fibrillarin in the fission yeast Schizosaccharomyces pombe and we have followed its expression under various conditions. Fission yeast fibrillarin is a 305 amino-acid protein which appears to be highly conserved throughout evolution. In Xenopus, human or Saccharomyces cerevisiae, a single fibrillarin mRNA is detected while, in S.pombe a single copy gene encodes different mRNAs which differ at the 3' ends. Under normal growth conditions, two mRNAs of 1.1 and 1.35 kb are detected with the 1.1 kb being the most abundant. Both the total amount and relative abundance of these two mRNAs are strongly affected by exposure to low temperature, namely the 1.1 kb mRNA almost disappears while the 1.35 kb is less markedly diminished. A new species of 3.2 kb accumulates in the cell, which contains an unusually long 3' untranslated region of 2 kb. We have found that exposure of the cells to a cold shock has a profound effect on 3' end formation in S.pombe since the transcription of several other mRNAs is also capable of skipping the normal 3' end site to terminate at a further downstream site.