Mesoporous Silica Supported Silver-Bismuth Nanoparticles as Photothermal Agents for Skin Infection Synergistic Antibacterial Therapy
Mesoporous Silica Supported Silver-Bismuth Nanoparticles as Photothermal Agents for Skin Infection Synergistic Antibacterial Therapy
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DOI：
10.1002/smll.202000436
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发表时间：
2020-05-13
期刊：
影响因子：
13.3
通讯作者：
Dong, Xiaochen
中科院分区：
文献类型：
--
作者：
Cao, Changyu;Ge, Wei;Dong, Xiaochen
多重耐药菌的出现导致了大量顽固的医院感染，严重威胁着人类的健康。迫切需要开发新的杀菌剂和治疗策略。本论文构建了介孔二氧化硅负载银-铋纳米粒子(Ag-Bi@SiO_2纳米粒子)，用于协同抗菌治疗。体外实验表明，BiNPs引发的高温可以破坏细胞的完整性，加速银离子的释放，进而对耐甲氧西林金黄色葡萄球菌(MRSA)表现出良好的抗菌性能。此外，在激光照射下，100µg·L~(-1)的Ag-Bi@SiO_2纳米粒子能有效地清除成熟的MRSA生物膜，使生物量减少69.5%，治疗效果优于加激光处理的纳米粒子(26.8%)或未经激光处理的纳米粒子(30.8%)。更重要的是，体内结果证实，使用银-铋@二氧化硅纳米粒子抗菌平台，脓肿中约95.4%的细菌被杀灭，脓肿消融速度加快。因此，具有光热增强抗菌活性的银-铋@二氧化硅纳米粒子是一种潜在的治疗皮肤感染的纳米抗菌剂。
The emergence of multidrug resistant bacteria has resulted in plenty of stubborn nosocomial infections and severely threatens human health. Developing novel bactericide and therapeutic strategy is urgently needed. Herein, mesoporous silica supported silver-bismuth nanoparticles (Ag-Bi@SiO2 NPs) are constructed for synergistic antibacterial therapy. In vitro experiments indicate that the hyperthermia originating from Bi NPs can disrupt cell integrity and accelerate the Ag ions release, further exhibiting an excellent antibacterial performance toward methicillin-resistant Staphylococcus aureus (MRSA). Besides, under laser irradiation, Ag-Bi@SiO2 NPs at 100 mu g mL(-1) can effectively obliterate mature MRSA biofilm and cause a 69.5% decrease in the biomass, showing a better therapeutic effect than Bi@SiO2 NPs with laser (26.8%) or Ag-Bi@SiO2 NPs without laser treatment (30.8%) groups. More importantly, in vivo results confirm that approximate to 95.4% of bacteria in abscess are killed and the abscess ablation is accelerated using the Ag-Bi@SiO2 NPs antibacterial platform. Therefore, Ag-Bi@SiO2 NPs with photothermal-enhanced antibacterial activity are a potential nano-antibacterial agent for the treatment of skin infections.