Enzymatically-controlled biomimetic synthesis of titania/protein hybrid thin films.
Enzymatically-controlled biomimetic synthesis of titania/protein hybrid thin films.
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DOI：
10.1039/c8tb00381e
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发表时间：
2018-06
期刊：
影响因子：
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通讯作者：
L. Bawazer;J. Ihli;M. Levenstein;L. Jeuken;F. Meldrum;D. G. McMillan
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文献类型：
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作者：
L. Bawazer;J. Ihli;M. Levenstein;L. Jeuken;F. Meldrum;D. G. McMillan
虽然人们普遍认为酶在塑造海绵中复杂的二氧化硅骨架方面发挥着重要作用，但在材料合成中利用酶的潜力尚未得到充分利用。在这项工作中，我们表明消化酶木瓜蛋白酶可以在氧化物表面自组装成单层，然后驱动晶体二氧化钛纳米颗粒的形成。这种薄膜形成和矿化促进的双重功能具有能够以连续无定形二氧化钛/蛋白质膜的形式构建分级无机/有机结构的潜力，所述连续无定形二氧化钛/蛋白质膜在退火后可以被精制至93%的结晶度。通过使用简单的浸涂方法的逐层处理提供对膜厚度的额外控制。木瓜蛋白酶的TiO 2矿化活性显示复杂的动力学，偏离了天然的Michaelis-Menten动力学活性，但失活研究表明，这种活性依赖于催化位点功能所必需的残基。这些参数为酶促生物矿化提供了独特的见解，允许实现生物工程二氧化钛异质结构的灵活途径，并可能为光伏电池开发提供绿色化学解决方案。
Although it is widely recognised that enzymes play a significant role in sculpting complex silica skeletons in marine sponges, the potential for exploiting enzymes in materials synthesis has not yet been fully harnessed. In this work we show that the digestive enzyme papain can self-assemble into monolayers on oxide surfaces, where they then drive the formation of crystalline titanium dioxide nanoparticles. This dual functionality of thin film formation and mineralization promotion has the potential to enable the construction of hierarchical inorganic/organic structures in the form of continuous amorphous titania/protein films which can be refined to 93% anatase post annealing. Additional control over the film thickness is afforded by layer-by-layer processing using a simple dip-coating approach. Papain's TiO2-mineralizing activity displays complex kinetics that deviates from the native Michaelis-Menten kinetic activity, yet deactivation studies demonstrate that this activity relies upon residues that are essential for catalytic site function. These parameters provide unique insight into enzymatic biomineralization, allowing a flexible route to achieving bioengineered titania heterostructures, and potentially providing a green-chemistry solution to photovoltaic cell development.