Study on the immobilization of anti-IgG on Au-colloid modified gold electrode via potentiometric immunosensor, cyclic voltammetry, and electrochemical impedance techniques
Study on the immobilization of anti-IgG on Au-colloid modified gold electrode via potentiometric immunosensor, cyclic voltammetry, and electrochemical impedance techniques
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DOI：
10.1016/j.colsurfb.2004.10.022
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发表时间：
2005-01-15
影响因子：
5.8
通讯作者：
Xu, L
中科院分区：
文献类型：
--
作者：
Fu, YZ;Yuan, R;Xu, L
本文研究了抗IgG在金胶体修饰金电极上的固定化。首先将清洁的金电极浸入巯基乙胺（AET）溶液中，然后将金纳米颗粒化学吸附到巯基乙胺的巯基基团上。终于来了抗-IgG被吸附到金纳米颗粒的表面上。采用电位免疫传感器、循环伏安法和电化学阻抗技术研究了抗IgG在Au胶体上的固定。在交流阻抗谱研究中，金胶体修饰电极与金纳米粒子修饰电极的电子传递电阻存在明显的差异。裸露的Bold电极暴露-随着抗IgG浓度的增加，循环伏安图趋于更不可逆。利用电位免疫传感器，所提出的技术是基于抗体包被的金纳米粒子的特异性凝集。平均直径为16 nm。在相应抗原的存在下引起电位变化，该电位变化由电位测定法监测。结果表明，所建立的免疫凝集试验系统对低至12 ng/mL的IgG抗原具有良好的灵敏度。实验结果表明，所开发的技术是在令人满意的协议与ELISA方法，金纳米粒子可用作抗体或抗原固定的生物相容性矩阵。(C)2004 Elsevier B. V.保留所有权利。
The immobilization of anti-IgG on Au-colloid modified gold electrodes has been investigated. A cleaned gold electrode was first immersed in a mercaptoethylamine (AET) solution, and then gold nanoparticles were chemisorbed onto the thiol groups of the mercaptoethylamine. Finally. anti-IgG was adsorbed onto the surface of the gold nanoparticles. Potentiometric immunosensor, cyclic voltammetry, and electrochemical impedance techniques were used to investigate the immobilization of anti-IgG on Au colloids. In the impedance spectroscopic study, an obvious difference of the electron transfer resistance between the Au-colloid modified electrode and the. bare Bold electrode seas observed-The cyclic voltammogram tends to be more irreversible with increased anti-IgG concentration. Using the potentiometric immunosensor, the proposed technique is based on that the specific agglutination of antibody-coated gold nanoparticles. averaging 16 nm in diameter. in the presence of the corresponding antigen causes a potential change that is monitored by a potentiometry. It is found that the developed immunoagglutination assay system is sensitive to the concentration of IgG antigen as low as 12 ng mL(-1). Experimental results showed that the developed technique is in satisfactory agreement with the ELISA method, and that gold nanoparticles can be used as a biocompatible matrix for antibody or antigen immobilization. (C) 2004 Elsevier B.V. All rights reserved.