A protocol for a highly consistent, high level production in vivo of Plasmodium falciparum oocysts and sporozoites.
A protocol for a highly consistent, high level production in vivo of Plasmodium falciparum oocysts and sporozoites.
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DOI：
10.4236/abb.2014.513112
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发表时间：
2014-01-01
期刊：
影响因子：
--
通讯作者：
Taylor-Robinson, A. W.
中科院分区：
文献类型：
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作者：
Looker, M.;Taylor-Robinson, A. W.
人类疟疾寄生虫恶性疟原虫和其按蚊媒介之间的密切关系的调查需要可靠的生产和分离的连续性阶段的寄生虫从受感染的蚊子。繁殖方面的这种进展将有利于一系列分子、细胞、免疫化学和传播阻断研究。寄生虫的培养、蚊子的饲养、感染和随后的蚊子解剖都是高度技术性的程序，需要技能和经验才能胜任。此外，为了在寄生虫可感染的短时期内产生适当年龄的蚊子以进行感染，需要精确的规划，以协调寄生虫和载体的相互作用的生命周期。在这里，一个协议是描述恶性疟原虫内斯氏按蚊的完整发展。通过分别解剖蚊子中肠和唾液腺，可以证明恶性疟原虫卵囊和子孢子在体内的非常一致的高水平产生。
Investigation of the intimate relationship between the human malaria parasite Plasmodium falciparum and its Anopheles vector requires the reliable production and isolation of successive sexual stages of the parasite from infected mosquitoes. Such an advance in propagation would benefit a range of molecular, cellular, immunochemical and transmission-blocking research studies. Parasite cultivation, mosquito rearing, infection and subsequent dissection of mosquitoes are all highly technical procedures that require both skill and experience to perform with competence. Furthermore, to produce mosquitoes of an appropriate age to infect during the short period in which parasites are viable for infection demands precise planning in order to coordinate the interacting life cycles of the parasite and vector. Here, a protocol is described for the complete development of P. falciparum within Anopheles stephensi. A very consistent, high level production in vivo of P. falciparum oocysts and sporozoites is demonstrable by dissection of the mosquito midgut and salivary glands, respectively.