Control of autophagosome size and number by Atg7
Control of autophagosome size and number by Atg7
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DOI：
10.1016/j.bbrc.2018.06.056
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发表时间：
2018-09-05
影响因子：
3.1
通讯作者：
Backues, Steven K.
中科院分区：
文献类型：
--
作者：
Cawthon, Hayley;Chakraborty, Ronith;Backues, Steven K.
在面包酵母中，氮饥饿诱导大量自噬。酿酒酵母）涉及许多自噬相关蛋白的上调，包括Atg 7。研究这种上调的重要性的一种方法是测量当该蛋白质的量不足时形成的自噬体的大小和数量。已知Atg8影响自噬体的大小，与其在吞噬细胞扩张中的作用一致。Atg7位于Atg8的上游，因此可能仅影响自噬体的大小。我们使用电子显微镜来测量有限数量的Atg 7形成的自噬体的大小和数量，发现它们比正常情况下更小更少。这表明，Atg7可能有一个Atg8独立的自噬体启动的作用，除了其Atg8依赖的自噬体扩展的作用。我们还提出了一个改进的模拟估计原始自噬体的数量的基础上观察到的横截面的横截面的切片。(C)2018爱思唯尔公司All rights reserved.
The induction of bulk autophagy by nitrogen starvation in baker's yeast (S. cerevisiae) involves the upregulation of many autophagy related proteins, including Atg7. One way to investigate the importance of this upregulation is to measure the size and number of autophagosomes formed when insufficient amounts of that protein are available. Atg8 is known to affect autophagosome size, consistent with its role in phagophore expansion. Atg7 is upstream of Atg8, and might therefore be expected to affect only autophagosome size. We used electron microscopy to measure the size and number of autophagosomes formed with limiting amounts of Atg7 and found them to be both smaller and fewer than normal. This suggests that Atg7 may have an Atg8-independent role in autophagosome initiation in addition to its Atg8-dependent role in autophagosome expansion. We also present an improved simulation for estimating original autophagic body number based on the number of cross-sections observed in ultrathin sections. (C) 2018 Elsevier Inc. All rights reserved.