DEFINING THE INSIDE AND OUTSIDE OF A CATALYTIC RNA MOLECULE
DEFINING THE INSIDE AND OUTSIDE OF A CATALYTIC RNA MOLECULE
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DOI：
10.1126/science.2501870
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发表时间：
1989-07-21
期刊：
影响因子：
56.9
通讯作者：
CECH, TR
中科院分区：
文献类型：
--
作者：
LATHAM, JA;CECH, TR
核酶是催化生物化学反应的RNA分子。Fe（II）-EDTA，一种溶剂型试剂，切割双链和单链RNA，被用来研究四膜虫核酶的结构。沿着整个RNA分子，切割区域与基本保护区域沿着交替。特别地，大部分催化核显示出大大降低的裂解。这些数据构成了实验证据，证明RNA酶像蛋白质酶一样，具有内部和外部。确定RNA的磷酸二酯骨架在分子内部或外部的位置为四膜虫核酶的三维结构建模提供了主要限制。这种方法通常应该为结构化RNA分子提供信息。
Ribozymes are RNA molecules that catalyze biochemical reactions. Fe(II)-EDTA, a solvent-based reagent which cleaves both double- and single-stranded RNA, was used to investigate the structure of the Tetrahymena ribozyme. Regions of cleavage alternate with regions of substantial protection along the entire RNA molecule. In particular, most of the catalytic core shows greatly reduced cleavage. These data constitute experimental evidence that an RNA enzyme, like a protein enzyme, has an interior and an exterior. Determination of positions where the phosphodiester backbone of the RNA is on the inside or on the outside of the molecule provides major constraints for modeling the three-dimensional structure of the Tetrahymena ribozyme. This approach should be generally informative for structured RNA molecules.