Spatiotemporal sequestration of miR165/166 by Arabidopsis Argonaute10 promotes shoot apical meristem maintenance.
Spatiotemporal sequestration of miR165/166 by Arabidopsis Argonaute10 promotes shoot apical meristem maintenance.
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DOI：
10.1016/j.celrep.2015.02.047
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发表时间：
2015-03
期刊：
影响因子：
8.8
通讯作者：
Yuyi Zhou;Minami Honda;Hongliang Zhu;Zhonghui Zhang;Xinwei Guo;Tianhong Li;Zhaohu Li;Xu Peng;
中科院分区：
文献类型：
--
作者：
Yuyi Zhou;Minami Honda;Hongliang Zhu;Zhonghui Zhang;Xinwei Guo;Tianhong Li;Zhaohu Li;Xu Peng;
拟南芥Argonaute10 (AGO10) 特异性地隔离miR165 和miR166 并拮抗它们的活性，从而调节茎尖分生组织(SAM) 的发育。然而，目前尚不清楚这种隔离作用的地点和时间。我们在此表明，AGO10 在早期胚胎发生过程中抑制胚胎本身的 miR165/166 活性，通过成熟胚胎的顶端和中央区域，并最终抑制子叶和叶原基的整个近轴结构域和脉管系统。这些位置与表达 miR165/166 的 mRNA 靶标 PHABULOSA 和 REVOLUTA 的区域基本相同。拟南芥基因组包含九个 MIR165/166 基因。 MIR165b、MIR166a、MIR166b 和 MIR166g 启动子对 miR165/166 的隔离有效地挽救了ago10突变体中的 SAM 缺陷。 AGO10 和四个 MIR165/166 成员的表达模式的比较表明，AGO10 抑制任何进入 AGO10 表达生态位的 miR165/166 的非细胞自主活性。因此，本研究深入了解 AGO10-miR165/166 活性的时空调节如何影响 SAM 发育。
ArabidopsisArgonaute10 (AGO10) specifically sequesters miR165 and miR166 and antagonizes their activity, thus regulating shoot apical meristem (SAM) development. However, where and when this sequestration acts is currently unclear. We show here that AGO10 represses miR165/166 activity in the embryo proper during early embryogenesis, through the apical and central regions of mature embryos, and eventually in the entire adaxial domain and vasculature of the cotyledons and leaf primordia. These locations are essentially identical to regions expressingPHABULOSAandREVOLUTA, mRNA targets of miR165/166. TheArabidopsisgenome contains nineMIR165/166genes. Sequestration of miR165/166 by theMIR165b,MIR166a,MIR166b, andMIR166gpromoters efficiently rescues the SAM defect inago10mutants. Comparison of the expression patterns ofAGO10and the fourMIR165/166members suggests that AGO10 quenches the non-cell-autonomous activity of any miR165/166 that moves intoAGO10-expressing niches. Thus, this study provides insight into how the spatiotemporal regulation of AGO10-miR165/166 activity affects SAM development.