Imiquimod does not affect shedding of viable chlamydiae in a murine model of Chlamydia trachomatis genital tract infection.
Imiquimod does not affect shedding of viable chlamydiae in a murine model of Chlamydia trachomatis genital tract infection.
复制标题
DOI：
10.1080/10647440300025503
复制
发表时间：
2003
影响因子：
--
通讯作者：
Ault KA
中科院分区：
文献类型：
--
作者：
Ramsey KH;Shaba N;Cohoon KP;Ault KA
目的：我们假设在小鼠模型中，口服或阴道给药免疫反应调节剂咪喹莫特会减少沙眼衣原体、小鼠肺炎菌株 (MoPn) 的阴道脱落。方法：雌性 BALB/c 小鼠阴道内感染 C.沙眼衣原体 (MoPn) 并在感染前和感染后每隔一天口服（30 mg/kg）或阴道（10 μl 5%咪喹莫特乳膏）给予咪喹莫特，总共四剂。通过收集宫颈阴道拭子并在 HeLa 229 细胞培养物中分离来监测感染过程。为了确定药物是否影响 1 型辅助性 T 细胞或 2 型辅助性 T 细胞免疫反应极化，在感染后第 56 天评估了免疫球蛋白 G (IgG) 亚类抗体反应。结果：无论是口服还是阴道给药，咪喹莫特治疗的小鼠与假治疗的对照组相比，感染过程没有显着差异。 IgG 亚类抗体反应，以及推而广之，T 辅助细胞 1 型到 T 辅助细胞 2 型免疫反应极化，也未受影响。结论：咪喹莫特对控制C.没有效果。小鼠模型中的沙眼衣原体（MoPn）感染。
Objective: We postulated that either oral or vaginal administration of the immune response modifier imiquimod would decrease vaginal shedding of Chlamydia trachomatis, mouse pneumonitis strain (MoPn), in a murine model. Methods: Female BALB/c mice were infected intravaginally withC. trachomatis (MoPn) and were administered imiquimod either orally (30 mg/kg) or vaginally (10 μl of 5%imiquimod cream) prior to infection and every second day after infection for a total of four doses. The course of infection was monitored by collecting cervical–vaginal swabs and isolation in HeLa 229 cell culture. To determine whether the drug affected T helper type 1 or T helper type 2 immune response polarization, immunoglobulinG(IgG) subclass antibody responses were assessed at day 56 after infection. Results: There was no significant difference in the course of infection when imiquimod-treated mice were compared with sham-treated controls, regardless of whether the drug was administered orally or vaginally. IgG subclass antibody responses, and by extension, T helper type 1 to T helper type 2 immune response polarization, were also unaffected. Conclusions: Imiquimod has no efficacy in controllingC. trachomatis (MoPn) infection in the murine model.