An evaluation of immunofluorescence and PCR methods for detection of rabies in archival Carnoy-fixed, paraffin-embedded brain tissue.
An evaluation of immunofluorescence and PCR methods for detection of rabies in archival Carnoy-fixed, paraffin-embedded brain tissue.
复制标题
对免疫荧光和 PCR 方法检测档案卡诺固定石蜡包埋脑组织中狂犬病的评估。
DOI：
10.1046/j.1439-0450.1999.00200.x
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Cynthia K. Warner
中科院分区：
文献类型：
--
作者：
K. Kulonen;Makonnen Fekadu;Sylvia G. Whitfield;Cynthia K. Warner
直接免疫荧光和PCR检测方法进行了比较的敏感性，在评估档案标本的卡诺伊固定，石蜡包埋的脑组织狂犬病的状态。这些材料包括1988年芬兰狂犬病爆发期间获得的23个样本，以及1985年在芬兰死于狂犬病的蝙蝠研究人员的一个样本。这些结果与新鲜组织上进行的原始诊断进行了比较。免疫荧光法检测狂犬病阳性档案病例的检出率为100%（12/12）。设计用于检测狂犬病核蛋白基因5'端附近的139-bp靶标的PCR检测法也检测到100%（12/12）新鲜组织标本中鉴定为阳性的样品。设计用于检测跨越第一次检测的139-bp靶标的304-bp靶标的PCR检测仅检测到67%（8/12）的原始病例。未记录假阳性。抗原的免疫荧光检测和核蛋白基因的短区域的PCR检测在确定固定的、石蜡包埋的（存档的）材料的狂犬病状态中是有用的。
Direct immunofluorescence and PCR detection methods were compared for sensitivity in evaluating the rabies status of archival specimens of Carnoy-fixed, paraffin-embedded brain tissue. The material consisted of 23 samples obtained during a rabies outbreak in Finland in 1988, and one sample isolated from a bat researcher who died of rabies in Finland in 1985. These results were compared with the original diagnoses performed on the fresh tissues. The immunofluorescence assay detected 100% (12/12) of the rabies-positive archival cases. A PCR assay designed to detect a 139-bp target near the 5' end of the rabies nucleoprotein gene also detected 100% (12/12) of the samples identified as positive in the fresh tissue specimens. A PCR assay designed to detect a 304-bp target spanning the 139-bp target of the first assay detected only 67% (8/12) of the original cases. No false positives were recorded. Both immunofluorescence detection of antigen and PCR detection of a short region of the nucleoprotein gene are useful in determining the rabies status of fixed, paraffin embedded (archival) material.