Characterization of murine BCAT genes:: Bcat1, a c-Myc target, and its homolog, Bcat2
Characterization of murine BCAT genes:: Bcat1, a c-Myc target, and its homolog, Bcat2
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DOI：
10.1007/s003359900825
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发表时间：
1998-07-01
期刊：
影响因子：
2.5
通讯作者：
Benvenisty, N
中科院分区：
文献类型：
--
作者：
Ben-Yosef, T;Eden, A;Benvenisty, N
原癌基因myc家族与基因转录调控有关(综述见Amati和Land1994)。所有Myc蛋白都含有几个已知转录因子的特征基序，并与另一种核蛋白Max一起，结合一个特定的DNA序列(Blackwell等人)。1990年；布莱克伍德和艾森曼1991年)。C-Myc诱导细胞过程的途径仍然不清楚，Myc活性的靶点也很大程度上是未知的。然而，在过去的几年里，一些基因被认为是c-Myc直接调控的(Schmidt 1996)。ECA39(HGMW批准的名称：BCAT1)是通过与c-Myc诱导的转基因小鼠肿瘤的消减/共表达策略分离出来的，并被证明是c-Myc调控的直接遗传靶点(Benvenisty等人)。(1992年)。BCAT1基因在胚胎发育早期高度表达，在器官发生过程中其表达定位于神经管、体节和中肾小管(Benvenisty等人)。(1992年)。该基因在几种基于c-myc的肿瘤中也有表达。最近，我们分离并比较了小鼠、人、线虫和酵母中BCAT1同源物的结构序列(Schuldiner等人。1996年)，并表明在人类中，就像在老鼠中一样，BCAT1基因是肿瘤发生过程中c-Myc活性的目标(Ben-Yosef等人。1996年)。最近，我们在人和酵母中鉴定了一个类似于BCAT1的基因，命名为ECA40(HGMW批准的名称：Bcat2)(Eden等人。1996年)。我们已经证明了这两个酵母同源物编码线粒体和胞质支链氨基酸氨基转移酶(BCAT；Eden等人)。1996年)。
The myc family of proto-oncogenes has been implicated in the regulation of gene transcription (for a review see Amati and Land 1994). All Myc proteins harbor several motifs characteristic of known transcription factors, and together with another nuclear protein, Max, they bind a specific DNA sequence (Blackwell et al. 1990; Blackwood and Eisenman 1991). The pathways by which c-Myc induces cellular processes are still obscure, and targets for Myc activity are largely unknown. Yet, in the past years, several genes have been suggested to be directly regulated by c-Myc (Schmidt 1996).The gene ECA39 (HGMW approved name: Bcat1) was isolated by a subtraction/co-expression strategy with c-Myc-induced tumors of transgenic mice, and proved to be a direct genetic target for c-Myc regulation in the mouse (Benvenisty et al. 1992). The Bcat1 gene is highly expressed early in embryogenesis, and during organogenesis its expression is localized to the neural tube, the somites, and the mesonephrous tubules (Benvenisty et al. 1992). This gene is also expressed in several c-myc-based tumors. Recently, we isolated and compared the structural sequences of the Bcat1 homologs in mouse, human, nematode, and yeast (Schuldiner et al. 1996) and showed that in human, as in mouse, the BCAT1 gene is a target for c-Myc activity in the oncogenesis process (Ben-Yosef et al. 1996). Lately, we have characterized a gene similar to BCAT1, named ECA40 (HGMW approved name: Bcat2), in both human and yeast (Eden et al. 1996). We have demonstrated that the two yeast homologs code for mitochondrial and cytosolic branched-chain amino acid aminotransferases (BCATs; Eden et al. 1996).