Functional micropatterned surfaces by combination of plasma polymerization and lift-off processes
Functional micropatterned surfaces by combination of plasma polymerization and lift-off processes
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DOI：
10.1002/ppap.200500071
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发表时间：
2006-01-16
影响因子：
3.5
通讯作者：
Rossi, F
中科院分区：
文献类型：
--
作者：
Brétagnol, F;Ceriotti, L;Rossi, F
在这项研究中，微结构表面是通过等离子体聚合和光刻相结合，通过不同功能域的空间排列来产生的。两种不同的蛋白质和细胞粘附图案与非污染区域交替出现。防污图案由聚环氧乙烷 (PEO) 类聚合物制成，该聚合物通过二甘醇二甲醚的脉冲等离子体聚合获得，从而形成具有高浓度环氧乙烷基团 (> 70%) 的涂层。污垢表面由具有低浓度环氧乙烷基团（约40%）的PEO涂层和通过等离子体聚合获得的含有氨基基团（来自烯丙胺单体）的薄膜组成。椭圆偏振测量证明了高图案保真度，而 XPS 和 ToF-SIMS 分析已用于表征表面。在图案表面上使用模型蛋白质（牛血清白蛋白）和细胞（L929 小鼠成纤维细胞）进行的实验表明，蛋白质和细胞仅粘附在图案上，而背景保持未覆盖。
In this study, microstructured surfaces are produced by a spatial arrangement of different functional domains by a combination of plasma polymerization and photolithography. Two different kinds of protein and cell adhesive patterns have been alternated with non-fouling areas. Nonfouling patterns are made of poly(ethylene oxide) (PEO)-like polymers obtained by pulsed plasma polymerization of diethylene glycol dimethyl ether, which leads to coatings with a high concentration of ethylene oxide groups (> 70%). Fouling surfaces are composed of PEO coatings with a low concentration of ethylene oxide groups (approximate to 40%) and films containing amino groups (from allylamine monomer) obtained by plasma polymerization. High pattern fidelity is demonstrated by ellispometry measurements, whereas XPS and ToF-SIMS analyses have been used to characterize the surfaces. Experiments with a model protein (bovine serum albumin) and cells (L929 mouse fibroblasts) on patterned surfaces show that proteins and cells only adhere on the patterns, whereas the background stays uncovered.