Membrane protein dynamics and functional implications in mammalian cells.
Membrane protein dynamics and functional implications in mammalian cells.
复制标题
哺乳动物细胞中的膜蛋白动力学和功能意义。
DOI：
10.1016/b978-0-12-417027-8.00003-9
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发表时间：
2013
影响因子：
--
通讯作者：
Golan, David E.
中科院分区：
文献类型：
--
作者：
Alenghat, Francis J.;Golan, David E.
质膜的组织是高度复杂和高度动态的。这种动态复杂性的一个表现是蛋白质在膜平面内的横向移动，这通常是分子间蛋白质结合作用、下游信号转导和局部膜机制的重要决定因素。膜蛋白的运动方式可以是随机布朗运动，也可以是静止运动，也可以是受限运动，也可以是主动定向运动。可以使用几种方法来区分蛋白质迁移率的不同模式，包括光漂白后的荧光恢复、单粒子跟踪、荧光相关光谱以及这些技术的变体。在这里，我们简要概述了这些方法和它们用于阐明哺乳动物细胞膜蛋白动态的例子--首先是在红细胞中，然后是在造血系中的红细胞和其他细胞中，最后是在非造血细胞中。这种多系统的分析表明，细胞骨架经常通过直接结合作用稳定地锚定蛋白质，通过空间相互作用限制蛋白质扩散，或者通过促进定向蛋白质运动来控制膜蛋白质的运动模式。总之，这些研究已经开始描述膜蛋白动力学影响信号后遗症和膜机械特性的机制，这反过来又控制细胞功能。
The organization of the plasma membrane is both highly complex and highly dynamic. One manifestation of this dynamic complexity is the lateral mobility of proteins within the plane of the membrane, which is often an important determinant of intermolecular protein-binding interactions, downstream signal transduction, and local membrane mechanics. The mode of membrane protein mobility can range from random Brownian motion to immobility and from confined or restricted motion to actively directed motion. Several methods can be used to distinguish among the various modes of protein mobility, including fluorescence recovery after photobleaching, single-particle tracking, fluorescence correlation spectroscopy, and variations of these techniques. Here, we present both a brief overview of these methods and examples of their use to elucidate the dynamics of membrane proteins in mammalian cells—first in erythrocytes, then in erythroblasts and other cells in the hematopoietic lineage, and finally in non-hematopoietic cells. This multisystem analysis shows that the cytoskeleton frequently governs modes of membrane protein motion by stably anchoring the proteins through direct-binding interactions, by restricting protein diffusion through steric interactions, or by facilitating directed protein motion. Together, these studies have begun to delineate mechanisms by which membrane protein dynamics influence signaling sequelae and membrane mechanical properties, which, in turn, govern cell function.